首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠鸟氨酸氨甲酰基转移酶，线粒体(OTC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的OTC含量。试剂盒检测范围为3.12 mU/mL-200 mU/mL，适用于肝病模型研究、尿素循环代谢机制探索、基因工程动物模型验证等科研场景，可满足科研实验中定量分析OTC酶动态变化的需求，为代谢性疾病机制研究、药物肝毒性评估等提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Otc ELISA Kit; Ornithine carbamoyltransferase ELISA Kit; mitochondrial ELISA Kit; EC 2.1.3.3 ELISA Kit; Ornithine transcarbamylase ELISA Kit; OTCase ELISA Kit
缩写：OTC
Uniprot No.：P11725
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 mU/mL-200 mU/mL
灵敏度：0.78 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse OTC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 86-97 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of mouse OTC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 97-109 | ||||
| EDTA plasma (n=4) | 96 | 91-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected2002.3482.2722.3102.1961001.8261.7321.7791.665501.1761.2391.2081.094250.7080.7430.7260.61212.50.4090.4310.4200.3066.250.2570.2710.2640.1503.120.1990.2040.2020.08800.1150.1120.114 | ||||||
货期：3-5 working days
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