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Recombinant Human TNFAIP8 protein

  • 中文名: 肿瘤坏死因子α诱导蛋白8(TNFAIP8)重组蛋白
  • 别    名: TNFAIP8;Tumor necrosis factor alpha-induced protein 8-like protein 1
货号: PA1000-3228
Price: ¥询价
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点TNFAIP8
Uniprot No O95379
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间2-198aa
氨基酸序列HSEAEESKEVATDVFNSKNLAVQAQKKILGKMVSKSIATTLIDDTSSEVLDELYRVTREYTQNKKEAEKIIKNLIKTVIKLAILYRNNQFNQDELALMEKFKKKVHQLAMTVVSFHQVDYTFDRNVLSRLLNECREMLHQIIQRHLTAKSHGRVNNVFDHFSDCEFLAALYNPFGNFKPHLQKLCDGINKMLDEENI
预测分子量 49.9kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于TNFAIP8重组蛋白的3篇参考文献示例,涵盖其功能机制及疾病关联研究:

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1. **文献名称**:*TNFAIP8 promotes cell proliferation and invasion in esophageal squamous cell carcinoma through activating PKB*

**作者**:Zhang, L. et al.

**摘要**:研究通过重组TNFAIP8蛋白在食管鳞癌细胞中的过表达,发现其通过激活PKB/Akt信号通路增强细胞增殖和侵袭能力,提示TNFAIP8可能作为癌症治疗的潜在靶点。

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2. **文献名称**:*Recombinant TNFAIP8 suppresses apoptosis in macrophages by modulating PI3K/AKT signaling*

**作者**:Padmavathi, G. et al.

**摘要**:利用原核表达系统制备重组TNFAIP8蛋白,证明其通过PI3K/AKT通路抑制巨噬细胞凋亡,并减少促炎因子释放,揭示了其在炎症调控中的关键作用。

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3. **文献名称**:*Structural and functional characterization of TNFAIP8 reveals its role in lipid metabolism*

**作者**:Sun, H. et al.

**摘要**:通过X射线晶体学解析重组TNFAIP8蛋白的三维结构,结合体外实验证实其结合脂质分子并调节代谢相关基因表达,为TNFAIP8在代谢疾病中的作用提供分子机制支持。

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**备注**:以上文献为示例,实际引用时建议通过PubMed或Web of Science检索最新研究。若需具体文献,可补充关键词或研究领域进一步筛选。

背景信息

TNFAIP8 (tumor necrosis factor alpha-induced protein 8) is a conserved protein family implicated in regulating apoptosis, immune responses, and carcinogenesis. Initially identified as a TNFα-inducible gene in human endothelial cells, TNFAIP8 exhibits pleiotropic functions across cellular processes. Structurally, it contains a characteristic death effector domain (DED)-like fold, enabling interactions with signaling molecules involved in cell survival and death pathways. Studies suggest TNFAIP8 acts as an anti-apoptotic regulator by inhibiting caspase activity and modulating PI3K/Akt, NF-κB, and MAPK signaling cascades. It also plays roles in immune homeostasis, particularly in negatively regulating Toll-like receptor (TLR)-triggered innate immune responses and T-cell activation.

Recombinant TNFAIP8 protein, produced via bacterial or mammalian expression systems, serves as a critical tool for deciphering its molecular mechanisms. Purified recombinant proteins retain functional domains, enabling in vitro binding assays, structural analysis, and interaction studies with partners like RACK1 or LC3. Its role in cancer has drawn significant attention, as TNFAIP8 is frequently overexpressed in malignancies such as breast, lung, and gastrointestinal cancers, correlating with tumor progression, metastasis, and chemoresistance. Recombinant forms facilitate research into its oncogenic potential, including modulation of autophagy, epithelial-mesenchymal transition (EMT), and therapeutic targeting. Additionally, emerging evidence links TNFAIP8 polymorphisms to autoimmune diseases, positioning recombinant proteins as valuable reagents for developing diagnostic or therapeutic strategies. Ongoing research aims to clarify its dual roles in promoting cell survival and immune evasion, highlighting its potential as a biomarker or intervention target in inflammatory disorders and cancer.

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