Mouse R-spondin-1(RSPO1) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL020550MO

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：小鼠R脊椎蛋白1(RSPO1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的RSPO1含量。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于体外研究RSPO1在发育生物学、干细胞分化、肿瘤微环境或再生医学中的表达变化本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Rspo1R-spondin-1 ELISA kit; Cysteine-rich and single thrombospondin domain-containing protein 3 ELISA kit; Cristin-3 ELISA kit; mCristin-3 ELISA kit; Roof plate-specific spondin-1 ELISA kit
缩写：RSPO1
Uniprot No.：Q9Z132
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Developmental Biology
测定原理：quantitative
测定方法：Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse RSPO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-97
1:2	Average %	101
	Range %	96-105
1:4	Average %	93
	Range %	85-97
1:8	Average %	92
	Range %	84-96

回收率：

The recovery of mouse RSPO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	81-94
EDTA plasma (n=4)	107	101-111

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/mlOD1OD2AverageCorrected4002.1982.1132.1561.9862001.3121.2761.2941.1241000.8860.8440.8650.695500.5040.5310.5180.348250.4020.4240.4130.24312.50.3190.3330.3260.1566.250.2370.2310.2340.06400.1710.1690.170