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产品描述：人R脊椎蛋白2(RSPO2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的RSPO2含量。RSPO2是一个重要靶点。其背景上，在多种生理过程中发挥作用。研究机制方面，它参与调控信号通路，如Wnt信号通路，对细胞的增殖、分化和组织发育等有影响，在肿瘤等疾病的发生发展机制研究中受到关注。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外研究RSPO2在肿瘤微环境中的调控机制、干细胞分化模型中的信号通路分析，以及利用多种生物样本探索疾病发生发展相关的分子机制，为生命科学基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：RSPO2 ELISA Kit; UNQ9384/PRO34209 ELISA Kit; R-spondin-2 ELISA Kit; Roof plate-specific spondin-2 ELISA Kit; hRspo2 ELISA Kit
缩写：RSPO2
Uniprot No.：Q6UXX9
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Developmental Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human RSPO2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-91 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 94-102 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 86-95 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-98 | |||||
回收率：
| The recovery of human RSPO2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 86-95 | ||||
| EDTA plasma (n=4) | 95 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected101.9741.9131.9441.79251.2981.2771.2881.1362.50.7390.7540.7470.5951.250.4260.4150.4210.2690.6250.3010.2870.2940.1420.3120.2340.2510.2430.0910.1560.1980.1920.1950.04300.1540.1500.152 | ||||||
货期：3-5 working days
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