首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠R脊椎蛋白2(RSPO2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的RSPO2含量。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于体外定量分析小鼠模型或细胞实验中RSPO2的表达水平变化，为研究其在发育生物学、疾病机制或信号通路调控中的功能提供可靠工具。该产品严格用于科学研究，不适用于临床诊断或其他用途。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Rspo2R-spondin-2 ELISA kit; Cysteine-rich and single thrombospondin domain-containing protein 2 ELISA kit; Cristin-2 ELISA kit; mCristin-2 ELISA kit; Roof plate-specific spondin-2 ELISA kit
缩写：RSPO2
Uniprot No.：Q8BFU0
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Developmental Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse RSPO2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 92-102 | ||||||
| 1:2 | Average % | 91 | |||||
| Range % | 85-98 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 88-93 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 96-104 | ||||||
回收率：
| The recovery of mouse RSPO2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 87-92 | |||||
| EDTA plasma (n=4) | 106 | 103-110 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 20002.5222.6332.5782.472 10002.1232.2132.1682.062 5001.5791.5441.5621.456 2501.0200.9891.0050.899 1250.7440.6880.7160.610 62.50.4600.4320.4460.340 31.250.2030.2140.2090.103 00.1020.1090.106 | |||||||
货期：3-5 working days
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