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产品描述：猪Syndecan-1/CD138(SDC1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SDC1含量。猪Syndecan-1（CD138）是一种细胞表面硫酸乙酰肝素蛋白聚糖，参与细胞增殖、迁移和细胞基质相互作用。其表达异常与多种疾病相关，如癌症和炎症性疾病，研究其机制有助于开发新的治疗策略。试剂盒检测范围为5 ng/mL-320 ng/mL，该产品可应用于猪模型研究中SDC1蛋白的表达分析，如评估炎症性疾病进程、组织再生机制研究或作为特定病理状态的潜在生物标志物探索，仅限科研用途。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：SDC1
Uniprot No.：K7GNF5
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：5 ng/mL-320 ng/mL
灵敏度：1.25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig SDC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 87-102 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 80-98 | |||||
回收率：
| The recovery of pig SDC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 81-98 | ||||
| EDTA plasma (n=4) | 98 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected3202.4132.3952.4042.0941602.1142.0042.0591.749801.7621.6741.7181.408401.4341.3391.3871.077201.0020.9530.9780.668100.8660.8590.8630.55350.5350.5290.5320.22200.3220.2970.310 | ||||||
货期：3-5 working days
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