首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人多配体聚糖2(SDC2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SDC2含量。试剂盒检测范围为93.75 pg/mL-6000 pg/mL，科研领域可用于探究SDC2在肿瘤发生发展、组织修复或炎症反应中的分子机制，例如通过检测血清/血浆中SDC2的浓度变化研究其与疾病进程的关联性，或利用组织匀浆分析特定器官中该蛋白的表达特征。满足基础研究及转化医学中对SDC2蛋白定量检测的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SDC2; HSPG1; Syndecan-2; SYND2; Fibroglycan; Heparan sulfate proteoglycan core protein; HSPG; CD antigen CD362
缩写：SDC2
Uniprot No.：P34741
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：93.75 pg/mL-6000 pg/mL
灵敏度：23.43 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SDC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||||||||
| Sample | Serum(n=4) | ||||||||||||
| 1:1 | Average % | 86 | |||||||||||
| Range % | 81-90 | ||||||||||||
| 1:2 | Average % | 98 | |||||||||||
| Range % | 93-101 | ||||||||||||
| 1:4 | Average % | 95 | |||||||||||
| Range % | 90-100 | ||||||||||||
| 1:8 | Average % | 94 | |||||||||||
| Range % | 86-97 | ||||||||||||
回收率：
| The recovery of human SDC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||||||||
| Sample Type | Average % Recovery | Range | |||||||||||
| Serum (n=5) | 94 | 90-97 | |||||||||||
| EDTA plasma (n=4) | 90 | 87-94 | |||||||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||
| pg/mlOD1OD2AverageCorrected60002.5082.4452.4772.31530001.8761.9191.8981.73615001.2941.1981.2461.0847500.7200.7030.7120.5503750.4650.4840.4750.313187.50.2620.2530.2580.09693.750.2110.2170.2140.05200.1640.1600.162 | |||||||||||||
货期：3-5 working days
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