Recombinant Human TSSC4 protein

中文名： 肿瘤抑制转移候选基因4(TSSC4)重组蛋白
别名： TSSC4；U5 small nuclear ribonucleoprotein TSSC4

货号: PA1000-3315

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>85%SDS-PAGE.
种属	Human
靶点	TSSC4
Uniprot No	Q9Y5U2
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-329aa
氨基酸序列	MAEAGTGEPS PSVEGEHGTE YDTLPSDTVS LSDSDSDLSL PGGAEVEALS PMGLPGEEDS GPDEPPSPPS GLLPATVQPF HLRGMSSTFS QRSRDIFDCL EGAARRAPSS VAHTSMSDNG GFKRPLAPSG RSPVEGLGRA HRSPASPRVP PVPDYVAHPE RWTKYSLEDV TEVSEQSNQA TALAFLGSQS LAAPTDCVSS FNQDPSSCGE GRVIFTKPVR GVEARHERKR VLGKVGEPGR GGLGNPATDR GEGPVELAHL AGPGSPEAEE WGSHHGGLQE VEALSGSVHS GSVPGLPPVE TVGFHGSRKR SRDHFRNKSS SPEDPGAEV
预测分子量	kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于TSSC4重组蛋白的3篇参考文献示例(注：TSSC4研究相对较少，以下内容为模拟文献示例)：

1. **"Functional characterization of TSSC4 recombinant protein in epigenetic regulation"**

*Authors: Zhang L, et al. (2020)*

摘要：研究通过大肠杆菌系统表达纯化TSSC4重组蛋白，发现其与组蛋白修饰复合体相互作用，可能通过调控H3K9甲基化抑制肿瘤细胞增殖。

2. **"Structural analysis of TSSC4 and its role in imprinting disorders"**

*Authors: Smith J, et al. (2018)*

摘要：利用重组TSSC4蛋白进行X射线晶体结构解析，揭示其N端结构域与PHD锌指蛋白的相互作用机制，为Beckwith-Wiedemann综合征相关研究提供结构基础。

3. **"TSSC4 recombinant protein modulates oxidative stress response in renal cells"**

*Authors: Gupta R, et al. (2016)*

摘要：通过哺乳动物细胞表达系统制备TSSC4重组蛋白，证明其能结合SOD1并增强肾小管细胞的抗氧化能力，提示其在急性肾损伤中的潜在保护作用。

(注：实际研究中TSSC4相关文献较少，建议通过PubMed或Google Scholar以"TSSC4 recombinant"或"TSSC4 protein"为关键词检索最新文献。若需具体文章，可提供更详细的研究背景。)

背景信息

TSSC4 (Tumor Suppressing Subtransferable Candidate 4) is a protein-coding gene located on human chromosome 11p15.5. a genomic region associated with imprinting disorders and cancer. It belongs to the Beckwith-Wiedemann syndrome (BWS) critical region, which harbors multiple imprinted genes involved in growth regulation. TSSC4 is paternally expressed and functions as a putative tumor suppressor, though its precise molecular mechanisms remain under investigation. Studies suggest its involvement in regulating apoptosis, cell proliferation, and epigenetic modifications, potentially through interactions with other imprinted genes like CDKN1C and PHLDA2.

Recombinant TSSC4 protein is engineered using expression systems (e.g., E. coli or mammalian cells) to produce purified, biologically active forms for functional studies. Its production enables researchers to explore protein-protein interactions, signaling pathways, and structural features. Notably, TSSC4 contains a conserved domain (DUF3448) of unknown function, motivating structural analysis via recombinant variants. Research applications include investigating its role in embryonic development, imprinting disorders like BWS, and cancer biology—particularly in tumors showing 11p15.5 alterations (e.g., Wilms’ tumor, hepatoblastoma). It’s also used to study metabolic regulation, as mouse models indicate TSSC4 deficiency impacts placental nutrient transport. Challenges in working with recombinant TSSC4 include maintaining proper folding due to its putative intrinsically disordered regions and reconciling conflicting data about its subcellular localization. Current studies focus on clarifying its tumor-suppressive mechanisms and therapeutic potential.