Recombinant Human UBE2E1 protein

中文名： 泛素结合酶E2E1(UBE2E1)重组蛋白
别名： UBE2E1；UBCH6；Ubiquitin-conjugating enzyme E2 E1

货号: PA1000-3369

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>95%SDS-PAGE.
种属	Human
靶点	UBE2E1
Uniprot No	P51965
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	2-193aa
氨基酸序列	SDDDSRASTSSSSSSSSNQQTEKETNTPKKKESKVSMSKNSKLLSTSAKR IQKELADITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDIT FTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLS ICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYAT
预测分子量	27 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于UBE2E1重组蛋白的3篇代表性文献(模拟示例，非真实文献)：

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1. **文献名称**: "Recombinant UBE2E1 Expression and Its Role in Ubiquitination Cascades"

**作者**: Smith A, et al.

**摘要**: 本研究报道了在大肠杆菌中高效表达并纯化UBE2E1重组蛋白的方法，通过体外泛素化实验证实其与E3连接酶Parkin的协同作用，揭示了UBE2E1在神经退行性疾病相关蛋白降解中的潜在功能。

2. **文献名称**: "Structural Insights into UBE2E1 Catalytic Mechanism by X-ray Crystallography"

**作者**: Chen L, et al.

**摘要**: 通过X射线晶体学解析了UBE2E1重组蛋白的3D结构，阐明了其活性位点关键氨基酸残基对泛素转移反应的调控机制，为开发靶向泛素化通路的小分子抑制剂提供结构基础。

3. **文献名称**: "UBE2E1-Mediated p53 Ubiquitination in Cancer Cell Proliferation"

**作者**: Tanaka K, et al.

**摘要**: 利用重组UBE2E1蛋白进行功能研究，发现其通过促进p53蛋白的泛素化降解调控细胞周期进程，在多种癌症细胞系中验证了UBE2E1过表达与肿瘤增殖的相关性。

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**备注**：以上内容为模拟生成，实际文献需通过PubMed、Web of Science等平台检索关键词“UBE2E1 recombinant protein”或“UBE2E1 ubiquitination”获取。

背景信息

**Background of UBE2E1 Recombinant Protein**

UBE2E1 (Ubiquitin-Conjugating Enzyme E2 E1) is a member of the E2 ubiquitin-conjugating enzyme family, which plays a pivotal role in the ubiquitin-proteasome system (UPS). This system regulates critical cellular processes, including protein degradation, signal transduction, and DNA repair, by tagging target proteins with ubiquitin molecules. UBE2E1 specifically transfers ubiquitin from E1 activating enzymes to substrate proteins, often in coordination with E3 ubiquitin ligases, marking them for proteasomal degradation or functional modulation.

Structurally, UBE2E1 contains a conserved catalytic core domain typical of E2 enzymes, enabling interactions with E1 and E3 enzymes. Dysregulation of UBE2E1 has been linked to various diseases, particularly cancers and neurodegenerative disorders. For instance, abnormal UBE2E1 expression may contribute to tumorigenesis by destabilizing tumor suppressors or promoting oncoprotein turnover, as observed in studies on hepatocellular carcinoma and colorectal cancer.

Recombinant UBE2E1 protein is produced via genetic engineering, often using bacterial or mammalian expression systems to ensure proper folding and post-translational modifications. This purified protein retains enzymatic activity and is widely used in *in vitro* ubiquitination assays, enzyme kinetics studies, and drug discovery research. It also aids in elucidating UBE2E1’s interaction networks, substrate specificity, and structural dynamics through techniques like X-ray crystallography or NMR.

Overall, UBE2E1 recombinant protein serves as a vital tool for decoding UPS mechanisms and developing therapeutic strategies targeting ubiquitination pathways in disease contexts. Its study continues to uncover novel insights into cellular homeostasis and pathology.