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Recombinant Human NCR3LG1 protein

  • 中文名: 天然细胞毒性触发受体3配体1(NCR3LG1)重组蛋白
  • 别    名: NCR3LG1;B7H6;Natural cytotoxicity triggering receptor 3 ligand 1
货号: PA1000-4033
Price: ¥询价
数量:
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产品详情

纯度>95%SDS-PAGE.
种属Human
靶点NCR3LG1
Uniprot NoQ68D85
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-262aa
氨基酸序列MTWRAAASTC AALLILLWAL TTEGDLKVEM MAGGTQITPL NDNVTIFCNI FYSQPLNITS MGITWFWKSL TFDKEVKVFE FFGDHQEAFR PGAIVSPWRL KSGDASLRLP GIQLEEAGEY RCEVVVTPLK AQGTVQLEVV ASPASRLLLD QVGMKENEDK YMCESSGFYP EAINITWEKQ TQKFPHPIEI SEDVITGPTI KNMDGTFNVT SCLKLNSSQE DPGTVYQCVV RHASLHTPLR SNFTLTAARH SLSETEKTDN FS
预测分子量28 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于NCR3LG1重组蛋白的3篇示例参考文献(注:部分内容为示例性概括,建议结合实际数据库检索获取准确文献):

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1. **文献名称**: *"Recombinant NCR3LG1 Protein Enhances NK Cell Cytotoxicity via Synergistic Receptor Activation"*

**作者**: Smith A, et al.

**摘要**: 本研究通过大肠杆菌表达系统成功制备了功能性NCR3LG1重组蛋白,验证其与自然杀伤细胞(NK细胞)表面受体的结合能力。实验表明,重组NCR3LG1可显著增强NK细胞对肿瘤细胞的杀伤活性,提示其在免疫治疗中的应用潜力。

2. **文献名称**: *"Structural and Functional Characterization of NCR3LG1 in Viral Infection Models"*

**作者**: Zhang Y, et al.

**摘要**: 利用哺乳动物细胞表达系统纯化NCR3LG1重组蛋白,结合晶体结构分析和体外感染模型,揭示了该蛋白通过调控干扰素通路抑制病毒复制的分子机制,为抗病毒药物开发提供新靶点。

3. **文献名称**: *"High-Yield Production of NCR3LG1 in HEK293 Cells for Therapeutic Antibody Screening"*

**作者**: Lee J, et al.

**摘要**: 开发了一种基于HEK293细胞的高效NCR3LG1重组蛋白生产平台,优化后的蛋白保留天然构象并用于筛选靶向抗体,成功鉴定出可阻断肿瘤免疫逃逸的中和性抗体候选分子。

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**建议**:若需真实文献,可通过PubMed或Google Scholar检索关键词“NCR3LG1 recombinant protein”、“NCR3LG1 expression”或结合具体研究领域(如免疫学、癌症治疗)进一步筛选。

背景信息

NCR3LG1 (Natural Cytotoxicity Triggering Receptor 3 Ligand 1), also known as B7-H6. is a cell surface protein belonging to the B7 family of immune regulatory molecules. It was first identified in 2009 as a ligand for the activating natural killer (NK) cell receptor NKp30 (NCR3), playing a critical role in NK cell-mediated immune responses. Structurally, NCR3LG1 is a type I transmembrane protein composed of an extracellular IgV-like domain, a transmembrane region, and a short cytoplasmic tail. Its expression is tightly regulated and primarily induced under pathological conditions, such as inflammation, infection, or cancer.

In normal tissues, NCR3LG1 is scarcely expressed but becomes upregulated on the surface of stressed, infected, or malignant cells. This upregulation enables NK cells to recognize and eliminate abnormal cells through NKp30 binding, triggering cytotoxic granule release and cytokine production. However, some tumors exploit NCR3LG1 overexpression to promote immune evasion by inducing NKp30 internalization and NK cell dysfunction, highlighting its dual role in immunity.

Recombinant NCR3LG1 protein is engineered to study these interactions, typically produced in mammalian expression systems to ensure proper glycosylation and functionality. Researchers use it to investigate NK cell activation mechanisms, tumor immune escape strategies, and potential therapeutic applications. In immunotherapy development, recombinant NCR3LG1 serves as a tool for enhancing NK cell cytotoxicity or designing blocking agents to counteract its immunosuppressive effects in cancers. Its role in viral infections (e.g., HIV, CMV) and autoimmune diseases is also under exploration, making it a versatile target in immunology research.

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