| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POSTN |
| Uniprot No | Q15063 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-781aa |
| 氨基酸序列 | NNHYDKILAHSRIRGRDQGPNVCALQQILGTKKKYFSTCKNWYKKSICGQ KTTVLYECCPGYMRMEGMKGCPAVLPIDHVYGTLGIVGATTTQRYSDASK LREEIEGKGSFTYFAPSNEAWDNLDSDIRRGLESNVNVELLNALHSHMIN KRMLTKDLKNGMIIPSMYNNLGLFINHYPNGVVTVNCARIIHGNQIATNG VVHVIDRVLTQIGTSIQDFIEAEDDLSSFRAAAITSDILEALGRDGHFTL FAPTNEAFEKLPRGVLERIMGDKVASEALMKYHILNTLQCSESIMGGAVF ETLEGNTIEIGCDGDSITVNGIKMVNKKDIVTNNGVIHLIDQVLIPDSAK QVIELAGKQQTTFTDLVAQLGLASALRPDGEYTLLAPVNNAFSDDTLSMD QRLLKLILQNHILKVKVGLNELYNGQILETIGGKQLRVFVYRTAVCIENS CMEKGSKQGRNGAIHIFREIIKPAEKSLHEKLKQDKRFSTFLSLLEAADL KELLTQPGDWTLFVPTNDAFKGMTSEEKEILIRDKNALQNIILYHLTPGV FIGKGFEPGVTNILKTTQGSKIFLKEVNDTLLVNELKSKESDIMTTNGVI HVVDKLLYPADTPVGNDQLLEILNKLIKYIQIKFVRGSTFKEIPVTVYRP TLTKVKIEGEPEFRLIKEGETITEVIHGEPIIKKYTKIIDGVPVEITEKE TREERIITGPEIKYTRISTGGGETEETLKKLLQEDTPVRKLQANKKVQGS RRRLREGRSQ |
| 预测分子量 | 86 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Periostin promotes the generation of fibrous tissues in pancreatic cancer"**
- **作者**: Takayama, G. et al.
- **摘要**: 研究通过重组POSTN蛋白揭示了其在胰腺癌微环境中促进纤维化基质形成的作用，增强肿瘤侵袭性和化疗抵抗。
2. **"Recombinant periostin enhances cardiac repair after myocardial infarction"**
- **作者**: Kudo, A. et al.
- **摘要**: 利用重组POSTN蛋白实验证明其通过激活整合素信号通路，促进心肌细胞迁移和瘢痕组织修复，改善心功能。
3. **"Recombinant periostin accelerates bone regeneration via modulation of osteoblast differentiation"**
- **作者**: Xie, C. et al.
- **摘要**: 在小鼠骨缺损模型中，重组POSTN蛋白通过调控Wnt/β-catenin通路显著促进成骨细胞分化和骨组织再生。
4. **"Targeting recombinant periostin in pulmonary fibrosis: mechanisms and therapeutic potential"**
- **作者**: Shibuya, Y. et al.
- **摘要**: 研究利用重组POSTN蛋白揭示其在肺纤维化中通过TGF-β信号促进肌成纤维细胞活化的机制，并评估其作为治疗靶点的潜力。
(注：以上文献信息为示例，实际引用需根据具体论文核实。)
**Background of POSTN Recombinant Protein**
Periostin (POSTN), also known as osteoblast-specific factor 2. is a secreted matricellular protein encoded by the *POSTN* gene. It plays a critical role in tissue remodeling, cell adhesion, and extracellular matrix (ECM) organization. Structurally, POSTN contains an N-terminal EMI domain, four tandem fasciclin-like (FAS-1) domains, and a C-terminal hydrophilic domain, enabling interactions with integrins (e.g., αvβ3. αvβ5), collagens, and other ECM components. These interactions facilitate cellular signaling pathways involved in wound healing, bone formation, and fibrotic responses.
POSTN is highly expressed in connective tissues under physiological conditions, such as during bone development and periodontal ligament maintenance. However, its dysregulation is linked to pathological processes, including cancer progression (e.g., metastasis, angiogenesis), cardiovascular fibrosis, and chronic inflammatory diseases (e.g., asthma, pulmonary fibrosis). In cancer, POSTN promotes tumor cell survival, invasion, and immune evasion by modulating the tumor microenvironment.
Recombinant POSTN protein is engineered using expression systems (e.g., mammalian, *E. coli*) to ensure proper folding and post-translational modifications. It is purified via affinity tags (e.g., His-tag) and validated for bioactivity using functional assays, such as cell migration or adhesion studies. Researchers utilize recombinant POSTN to investigate its role in cellular mechanisms, screen therapeutic targets, or model diseases in vitro and in vivo. Its application extends to studying regenerative medicine, as POSTN may enhance stem cell differentiation or tissue repair.
Overall, POSTN recombinant protein serves as a vital tool for unraveling the protein’s multifunctional roles and exploring its therapeutic potential in fibrosis, oncology, and tissue engineering.
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