| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ICAM1 |
| Uniprot No | P05362 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-480aa |
| 氨基酸序列 | QTSVSPSKVILPRGGSVLVTCSTSCDQPKLLGIETPLPKKELLLPGNNRK VYELSNVQEDSQPMCYSNCPDGQSTAKTFLTVYWTPERVELAPLPSWQPV GKNLTLRCQVEGGAPRANLTVVLLRGEKELKREPAVGEPAEVTTTVLVRR DHHGANFSCRTELDLRPQGLELFENTSAPYQLQTFVLPATPPQLVSPRVL EVDTQGTVVCSLDGLFPVSEAQVHLALGDQRLNPTVTYGNDSFSAKASVS VTAEDEGTQRLTCAVILGNQSQETLQTVTIYSFPAPNVILTKPEVSEGTE VTVKCEAHPRAKVTLNGVPAQPLGPRAQLLLKATPEDNGRSFSCSATLEV AGQLIHKNQTRELRVLYGPRLDERDCPGNWTWPENSQQTPMCQAWGNPLP ELKCLKDGTFPLPIGESVTVTRDLEGTYLCRARSTQGEVTRKVTVNVLSP RYE |
| 预测分子量 | 50 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ICAM1重组蛋白的3篇参考文献及其摘要概括：
1. **"Recombinant human ICAM-1 modulates inflammatory responses in endothelial cells"**
- 作者：Smith A et al.
- 摘要：研究通过重组人ICAM1蛋白处理内皮细胞，发现其能增强白细胞黏附并激活NF-κB通路，揭示ICAM1在炎症反应中的调控作用。
2. **"Expression and purification of functional ICAM-1 recombinant protein for ligand binding studies"**
- 作者：Zhang L et al.
- 摘要：开发了一种高效的大肠杆菌表达系统，成功纯化具有生物活性的ICAM1重组蛋白，并验证其与整合素LFA-1的结合能力。
3. **"ICAM-1 recombinant protein inhibits viral entry in a model of rhinovirus infection"**
- 作者：Johnson R et al.
- 摘要：体外实验表明，可溶性ICAM1重组蛋白通过竞争性结合鼻病毒颗粒，显著降低宿主细胞感染率，提示其作为抗病毒药物的潜力。
注：以上文献信息为示例，实际引用需根据具体研究查找真实发表的论文。
Intercellular Adhesion Molecule 1 (ICAM1), also known as CD54. is a transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily. It plays a critical role in mediating cell-cell interactions during inflammatory and immune responses. ICAM1 is expressed on endothelial cells, leukocytes, and various other cell types, and its expression is upregulated by pro-inflammatory cytokines such as TNF-α, IL-1. and IFN-γ. The protein interacts with leukocyte integrins, particularly LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18), facilitating leukocyte adhesion, transmigration across vascular endothelium, and immune synapse formation. Dysregulation of ICAM1 is implicated in chronic inflammation, autoimmune diseases, cancer progression, and viral infections (e.g., rhinoviruses utilize ICAM1 as a receptor).
Recombinant ICAM1 proteins are engineered to mimic the extracellular domain of native ICAM1. typically produced in mammalian expression systems (e.g., CHO or HEK293 cells) to ensure proper glycosylation and folding. These proteins often include the five Ig-like domains responsible for ligand binding. Researchers use recombinant ICAM1 in vitro to study leukocyte-endothelial interactions, screen anti-inflammatory drugs, or investigate pathogen-host interactions. In therapeutic contexts, soluble ICAM1 variants or ICAM1-targeting biologics are explored for modulating immune responses. Additionally, recombinant ICAM1 serves as a critical tool in structural biology to map binding interfaces and in diagnostic assays to detect autoantibodies in autoimmune disorders. Its versatility makes it valuable for both basic research and translational applications.
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