Recombinant Human PRM2 protein

中文名： 鱼精蛋白2(PRM2)重组蛋白
别名： PRM2；Protamine-2

货号: PA1000-4990

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	PRM2
Uniprot No	P04554
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-102aa
氨基酸序列	MVRYRVRSLSERSHEVYRQQLHGQEQGHHGQEEQGLSPEHVEVYERTHGQ SHYRRRHCSRRRLHRIHRRQHRSCRRRKRRSCRHRRRHRRGCRTRKRTCR RH
预测分子量	kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于PRM2(Protamine 2)重组蛋白研究的示例参考文献(注：以下文献信息为示例性质，非真实存在)：

1. **文献名称**: *Expression and purification of recombinant human PRM2 for functional studies*

**作者**: Smith A, et al.

**摘要**: 研究通过大肠杆菌表达系统成功表达并纯化了重组人PRM2蛋白，验证了其与DNA结合的能力，并探讨了其在精子染色质凝缩中的潜在作用。

2. **文献名称**: *Role of PRM2 in sperm chromatin stability: Insights from recombinant protein assays*

**作者**: Chen L, et al.

**摘要**: 利用重组PRM2蛋白进行体外实验，发现PRM2通过特异性磷酸化修饰调节染色质结构稳定性，突变体实验表明其功能异常可能导致男性不育。

3. **文献名称**: *Structural characterization of PRM2 recombinant protein by NMR spectroscopy*

**作者**: Johnson R, et al.

**摘要**: 采用核磁共振技术解析了重组PRM2蛋白的三维结构，揭示了其精氨酸富集区域与DNA相互作用的分子机制。

4. **文献名称**: *PRM2 deficiency and its rescue by recombinant protein supplementation in a mouse model*

**作者**: Wang Y, et al.

**摘要**: 在PRM2缺陷小鼠模型中，外源重组PRM2蛋白恢复了精子染色质完整性，证实其在生殖细胞发育中的关键作用。

(注：以上文献为模拟内容，实际研究中请通过学术数据库检索真实文献。)

背景信息

PRM2 (Protamine-2) is a small, arginine-rich nuclear protein critical for chromatin condensation during spermiogenesis, the final stage of sperm cell maturation. In mammals, protamines replace histones in elongating spermatids to compact paternal DNA into a transcriptionally inert state, ensuring genomic stability and protecting sperm DNA from damage. PRM2 is expressed as a precursor protein that undergoes post-translational processing, including phosphorylation and proteolytic cleavage, to generate its mature form. Its structural role involves neutralizing DNA negative charges via arginine residues, enabling tight chromatin packaging. Defects in PRM2 are linked to male infertility, abnormal sperm morphology, and DNA fragmentation in humans, while mouse models show complete male sterility upon PRM2 knockout.

Recombinant PRM2 protein is produced using biotechnological platforms (e.g., bacterial or mammalian expression systems) for functional studies and therapeutic development. Its production often involves codon optimization for heterologous expression, affinity tag fusion for purification, and verification of post-translational modifications through mass spectrometry. Research applications include investigating chromatin dynamics, DNA-protein interactions, and epigenetic regulation in reproduction. Clinically, recombinant PRM2 serves as a biomarker for male fertility diagnostics and a potential target for contraceptive development. Challenges remain in replicating its native phosphorylation patterns and understanding its non-structural roles in embryonic development. Current studies also explore its involvement in paternal epigenetic programming and transgenerational inheritance mechanisms.