关键信息
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基因名
CK19
- 应用
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别名
CK19；Keratin. type I cytoskeletal 19
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P08727
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表达区间
1-400aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSMTSYSYR QSSATSSFGG LGGGSVRFGP GVAFRAPSIH GGSGGRGVSV SSARFVSSSS SGAYGGGYGG VLTASDGLLA GNEKLTMQNL NDRLASYLDK VRALEAANGE LEVKIRDWYQ KQGPGPSRDY SHYYTTIQDL RDKILGATIE NSRIVLQIDN ARLAADDFRT KFETEQALRM SVEADINGLR RVLDELTLAR TDLEMQIEGL KEELAYLKKN HEEEISTLRG QVGGQVSVEV DSAPGTDLAK ILSDMRSQYE VMAEQNRKDA EAWFTSRTEE LNREVAGHTE QLQMSRSEVT DLRRTLQGLE IELQSQLSMK AALEDTLAET EARFGAQLAH IQALISGIEA QLGDVRADSE RQNQEYQRLM DIKSRLEQEI ATYRSLLEGQ EDHYNNLSAS KVL
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分子量
47 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Cytokeratin 19 (CK19) is a type of intermediate filament protein predominantly expressed in simple epithelial tissues, playing a crucial role in maintaining epithelial cell structural integrity and function. As a member of the keratin family, it is often utilized as a biomarker for various carcinomas, particularly in the diagnosis and prognosis of malignancies like breast, lung, and colorectal cancers. Research on recombinant CK19 proteins has gained traction, as these proteins can be produced in a controlled manner for various applications in cancer diagnostics and therapeutics. The recombinant form enables the study of CK19's biochemical properties, interactions with other proteins, and its role in tumorigenesis. Furthermore, understanding the structure-function relationship of CK19 through recombinant technologies can aid in the development of targeted therapies and personalized medicine approaches. By exploring the functionality of CK19 in vitro and in vivo, researchers aim to unveil the molecular mechanisms underlying epithelial cell behavior in both normal physiology and pathological conditions, thereby advancing the field of cancer research and improving clinical outcomes.
