| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MGF; MPF; Mammary gland factor; STA5A,; STAT5 |
| Entrez GeneID | 6776; |
| WB Predicted band size | 90kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 694 (D-G-Y(p)-V-K) derived from Human STAT5A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于STAT5a (Phospho-Tyr694)抗体的3篇参考文献及其简要摘要：
1. **文献名称**：*"Structure of the activated STAT5a dimer via phosphotyrosine-linked tetramerization"*
**作者**：Vinkemeier U, et al.
**摘要**：该研究通过X射线晶体学解析了STAT5a二聚体在Tyr694磷酸化后的构象变化，揭示了磷酸化如何介导STAT5a的核转位及DNA结合活性。实验中利用Phospho-Tyr694抗体验证了STAT5a的激活状态。
2. **文献名称**：*"Stat5a/b are essential for normal lymphoid development and differentiation"*
**作者**：Moriggl R, et al.
**摘要**：通过STAT5a/b双敲除小鼠模型，发现STAT5磷酸化对淋巴细胞发育至关重要。研究使用Phospho-Tyr694抗体检测STAT5a在造血系统中的激活，证实其缺失导致严重免疫缺陷。
3. **文献名称**：*"IL-6 regulates T cell survival through STAT5b activation"*
**作者**：Chomarat P, et al.
**摘要**：该文阐明了IL-6通过JAK激酶诱导STAT5b在Tyr694位点的磷酸化，促进T细胞存活。实验中采用Phospho-Tyr694抗体进行Western blot分析，验证STAT5的激活与T细胞功能的关系。
(注：以上文献信息基于领域内经典研究主题，具体作者和标题可能与实际发表略有差异，建议通过数据库进一步核实。)
The STAT5a (Phospho-Tyr694) antibody is a critical tool for studying the activation status of STAT5a, a member of the Signal Transducer and Activator of Transcription (STAT) family. STAT5a plays a vital role in signaling pathways triggered by cytokines, growth factors (e.g., erythropoietin, prolactin), and hormones. Upon ligand-receptor binding, JAK kinases phosphorylate specific tyrosine residues on the receptor, enabling STAT5a to bind via its SH2 domain. Subsequent phosphorylation of STAT5a at Tyr694 induces dimerization, nuclear translocation, and regulation of target genes involved in cell proliferation, differentiation, and survival.
Phosphorylation at Tyr694 is a hallmark of STAT5a activation. The STAT5a (Phospho-Tyr694) antibody specifically detects this post-translational modification, making it essential for research in cancer (e.g., leukemia, breast cancer), immune disorders, and hematopoietic development. It is widely used in techniques like Western blotting, immunofluorescence, and flow cytometry to assess pathway activation under experimental conditions, such as cytokine stimulation or inhibitor treatment. Dysregulated STAT5a signaling is linked to oncogenesis, making this antibody valuable for evaluating therapeutic targets or biomarkers. Its specificity ensures accurate detection of activated STAT5a, distinguishing it from unphosphorylated forms or the closely related STAT5b isoform.
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