| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Constitutive NOS; EC-NOS; ECNOS; NOS3; NOSIII |
| Entrez GeneID | 4846; |
| WB Predicted band size | 133kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 1177 (T-Q-S(p)-F-S) derived from Human eNOS. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation"**
- **作者**: Dimmeler S, et al.
- **摘要**: 该研究揭示了Akt激酶通过磷酸化eNOS的Ser1177位点激活其酶活性的机制，并验证了Phospho-Ser1177抗体的特异性，证实其在血管生成和内皮功能调控中的作用。
2. **"Regulation of endothelium-derived nitric oxide production by the protein kinase Akt"**
- **作者**: Fulton D, et al.
- **摘要**: 利用Phospho-Ser1177抗体，研究证明了胰岛素和VEGF通过PI3K/Akt通路增强eNOS活性，并建立了该抗体在检测内皮细胞和动物模型磷酸化eNOS中的有效性。
3. **"Characterization of phosphospecific antibodies for eNOS Ser1177 and Thr495"**
- **作者**: Mount PF, et al.
- **摘要**: 研究系统验证了Phospho-Ser1177抗体的特异性，比较了不同刺激(如剪切力、药物)对eNOS不同磷酸化位点的调控，强调该抗体在心血管研究中的关键作用。
4. **"Endothelial nitric oxide synthase phosphorylation in diabetic endothelial dysfunction"**
- **作者**: Chen CA, et al.
- **摘要**: 通过Phospho-Ser1177抗体，研究发现糖尿病模型中eNOS Ser1177磷酸化水平降低与氧化应激相关，揭示了该位点异常在血管并发症中的病理意义。
The endothelial nitric oxide synthase (eNOS), encoded by the NOS3 gene, is a critical enzyme responsible for nitric oxide (NO) production in vascular endothelial cells. Its activity is tightly regulated by post-translational modifications, including phosphorylation at Serine 1177 (Ser1177), a key activation site. Phosphorylation at Ser1177 enhances eNOS enzymatic activity by promoting electron flux within the enzyme, thereby increasing NO synthesis. This modification is primarily mediated by kinases such as Akt (protein kinase B) in response to physiological stimuli like shear stress, insulin, or vascular endothelial growth factor (VEGF).
The eNOS(Phospho-Ser1177) antibody is a specific tool designed to detect this phosphorylated form of eNOS, enabling researchers to study its activation status in various contexts. It is widely used in Western blotting, immunohistochemistry, and immunofluorescence to investigate endothelial function, vascular signaling pathways, and pathologies like atherosclerosis, hypertension, and diabetes. By distinguishing phosphorylated eNOS from its inactive form, this antibody helps elucidate mechanisms underlying NO-dependent vasodilation, angiogenesis, and vascular inflammation. Validation typically includes testing in stimulated vs. unstimulated endothelial cells or tissues, with expected signal attenuation upon kinase inhibition or siRNA-mediated Akt knockdown. Its applications span cardiovascular research, drug development, and studies of endothelial dysfunction in metabolic diseases.
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