| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KS6B1; P70-S6K; RPS6KB1; S6K; |
| Entrez GeneID | 6198; |
| WB Predicted band size | 70 85 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 411 (I-R-S(p)-P-R) derived from Human p70 S6 Kinase. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于p70S6激酶(Phospho-Ser411)抗体的示例参考文献(注：以下内容为模拟生成，实际文献需通过数据库验证)：
1. **"Phosphorylation of p70S6 Kinase at Ser411 Modulates mTORC1 Signaling in Response to Amino Acid Availability"**
- **作者**: Li et al.
- **摘要**: 本研究揭示了p70S6激酶Ser411位点的磷酸化在氨基酸信号调控mTORC1通路中的关键作用，并通过特异性抗体证实该磷酸化事件与细胞生长调控相关。
2. **"Development and Validation of a Phospho-Specific Antibody Targeting Ser411 on p70S6 Kinase"**
- **作者**: Chen & Park
- **摘要**: 文章报道了一种新型抗体的开发，该抗体特异性识别p70S6激酶Ser411磷酸化位点，并通过免疫印迹和免疫荧光验证其在多种细胞模型中的应用。
3. **"Phospho-Ser411-p70S6K as a Biomarker for Insulin Resistance in Adipose Tissue"**
- **作者**: Gupta et al.
- **摘要**: 利用Phospho-Ser411抗体，研究发现肥胖模型中p70S6激酶Ser411磷酸化水平升高，提示其与胰岛素抵抗及代谢紊乱的潜在关联。
4. **"Role of p70S6K Ser411 Phosphorylation in Cancer Cell Invasion"**
- **作者**: Rodriguez et al.
- **摘要**: 通过抑制实验及Phospho-Ser411抗体检测，本文证明该位点磷酸化与肿瘤细胞的侵袭能力增强相关，可能成为治疗靶点。
建议通过PubMed或Google Scholar检索关键词“p70S6 Kinase Ser411 phosphorylation antibody”获取真实文献。
The p70 S6 kinase (Phospho-Ser411) antibody is a crucial tool for studying the mTOR (mammalian target of rapamycin) signaling pathway, which regulates cell growth, proliferation, and survival. p70 S6 kinase (p70S6K), a serine/threonine kinase, exists in two isoforms (p70 and p85) derived from the same gene via alternative splicing. Phosphorylation at Ser411. located within its autoinhibitory domain, is a key regulatory event. This modification is mediated by mTOR complex 1 (mTORC1) in response to growth factors, nutrients, or energy sufficiency, leading to kinase activation. Once active, p70S6K phosphorylates ribosomal protein S6. enhancing translation of mRNA transcripts involved in ribosome biogenesis and cell cycle progression.
The p70S6K (Phospho-Ser411) antibody specifically detects this phosphorylation event, enabling researchers to assess mTORC1 activity in cells or tissues. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study signaling dynamics in conditions such as cancer, metabolic disorders, and neurodegenerative diseases. Validation often involves knockout controls or phosphatase treatment to confirm specificity. Aberrant phosphorylation at Ser411 has been linked to pathological states, including tumorigenesis and insulin resistance, making this antibody valuable for both basic research and therapeutic target validation. Proper experimental controls are essential to avoid cross-reactivity with related phospho-epitopes or isoforms.
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