| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CX43; CXA1; CXN-43; GJA1; |
| Entrez GeneID | 2697; |
| WB Predicted band size | 43kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 368 (R-A-S(p)-S-R) derived from Human Connexin 43. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于Connexin 43 (phospho-Ser368)抗体的代表性文献摘要：
1. **标题**: *Protein kinase C phosphorylation of connexin43 at Ser368 disrupts gap junctional communication*
**作者**: Solan JL, Lampe PD
**摘要**: 该研究证明PKC介导的Cx43 Ser368磷酸化会减少细胞间通讯，通过磷酸化特异性抗体证实该位点修饰导致间隙连接功能抑制。
2. **标题**: *Ischemia-induced phosphorylation of connexin43 at Ser368 contributes to gap junction internalization in cardiomyocytes*
**作者**: Hund TJ, Lerner DL, et al.
**摘要**: 利用phospho-Ser368抗体发现，心肌缺血时Cx43在此位点的磷酸化促进间隙连接内化，加剧心律失常风险。
3. **标题**: *Phosphorylation of connexin43 at Ser368 regulates assembly and function of cardiac gap junctions in heart failure*
**作者**: Xiao L, Ai J, et al.
**摘要**: 通过该抗体检测心力衰竭模型中Cx43异常磷酸化，揭示其导致心肌电传导障碍的分子机制。
提示：建议通过PubMed或Google Scholar以关键词“Connexin 43 Ser368 phosphorylation antibody”检索最新文献，部分研究可能涉及肿瘤微环境或细胞增殖调控。
Connexin 43 (Cx43), a gap junction protein encoded by the *GJA1* gene, facilitates direct intercellular communication by forming channels that allow the exchange of ions, metabolites, and signaling molecules. Post-translational modifications, particularly phosphorylation, dynamically regulate Cx43 function, localization, and stability. The phosphorylation at serine 368 (Ser368) is a critical regulatory event, often induced by protein kinase C (PKC) activation. This modification reduces channel permeability by promoting conformational changes or internalization of Cx43. thereby modulating intercellular communication under physiological or pathological conditions.
Antibodies targeting Cx43 phosphorylated at Ser368 (phospho-Ser368) are essential tools for studying this specific modification. They enable the detection of Cx43 activation states in response to cellular stimuli, such as stress, inflammation, or pharmacological agents. Researchers widely use these antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore Cx43 dynamics in tissues like the heart, brain, and skin. Dysregulated Cx43 phosphorylation at Ser368 has been implicated in cardiac arrhythmias, cancer progression, and impaired wound healing, making this antibody valuable for investigating disease mechanisms or therapeutic targets. Specificity validation via knockout controls or phosphatase treatment is recommended to ensure accurate interpretation of results.
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