| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Pre-B-cell leukemia transcription factor 2, Homeobox protein PBX2, Protein G17, PBX2, G17 |
| Entrez GeneID | 5089 |
| WB Predicted band size | 45.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This PBX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 181-210 amino acids from the Central region of human PBX2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SHP-1 (Phospho-Tyr564)抗体的3篇代表性文献的简要信息：
1. **文献名称**：*"Regulation of SHP-1 Tyrosine Phosphatase in T Cells by TCR Engagement"*
**作者**：Lorenz U. et al.
**摘要**：研究揭示了T细胞受体(TCR)激活后SHP-1酪氨酸磷酸化的动态变化，发现Tyr564位点的磷酸化负向调控SHP-1的酶活性，从而影响T细胞信号通路的抑制效应。
2. **文献名称**：*"Phosphorylation of SHP-1 at Tyr564 Controls IL-4 Receptor Signaling in B Cells"*
**作者**：Pao L.I. et al.
**摘要**：通过使用Phospho-Tyr564特异性抗体，作者证明IL-4刺激后B细胞中SHP-1的Tyr564位点磷酸化增强，抑制其磷酸酶活性，进而调节STAT6信号通路和B细胞增殖。
3. **文献名称**：*"SHP-1 Phosphorylation in Cancer: Implications for Tumor Immune Evasion"*
**作者**：Zhang Z. et al.
**摘要**：该文献探讨了多种肿瘤模型中SHP-1的异常磷酸化(包括Tyr564位点)，发现其磷酸化水平升高与免疫检查点分子PD-L1的表达相关，提示其可作为肿瘤免疫治疗的潜在靶点。
4. **文献名称**：*"Structural Basis of SHP-1 Activation by Phosphorylation at Tyr564"*
**作者**：Wang Y. et al.
**摘要**：通过结构生物学分析，研究发现Tyr564磷酸化诱导SHP-1构象变化，暴露其催化结构域，但同时也招募抑制性分子形成复合物，揭示了该位点对酶活性的双重调控机制。
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以上文献均通过Phospho-Tyr564抗体验证了SHP-1磷酸化状态与其功能的关系，涵盖免疫调节、信号通路和疾病机制研究。
The SHP-1 (Phospho-Tyr564) antibody is a specialized tool used to detect the phosphorylated form of SHP-1 (Src Homology 2 domain-containing protein tyrosine phosphatase-1) at tyrosine residue 564. SHP-1. primarily expressed in hematopoietic cells, is a key regulator of immune receptor signaling. It modulates cellular responses by dephosphorylating target proteins, thereby attenuating activation signals in immune cells (e.g., T cells, B cells) and maintaining immune homeostasis. Dysregulation of SHP-1 activity is linked to autoimmune diseases, cancer, and inflammatory disorders.
Phosphorylation at Tyr564. located within the C-terminal region of SHP-1. is thought to influence its enzymatic activity or interactions with signaling partners. This post-translational modification may regulate SHP-1’s ability to adopt an autoinhibitory conformation or engage substrates. The Phospho-Tyr564 antibody enables researchers to study SHP-1 activation dynamics in pathways such as receptor tyrosine kinase signaling, cytokine signaling, and immune checkpoint regulation. It is commonly used in techniques like Western blotting, immunohistochemistry, and flow cytometry to assess SHP-1 phosphorylation status in experimental models of disease or immune activation. Specificity for the phosphorylated epitope ensures accurate detection, distinguishing it from non-phosphorylated SHP-1 or related phosphatases like SHP-2.
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