Rabbit Polyclonal IKK-γ(Phospho-Ser376) Antibody

The IKK-γ (Phospho-Ser376) antibody is a specialized tool used to detect the phosphorylation of the IKK-γ protein (also known as NEMO, NF-κB essential modulator) at serine residue 376. IKK-γ is a critical regulatory subunit of the IκB kinase (IKK) complex, which governs the activation of the NF-κB signaling pathway—a central mediator of inflammation, immune responses, and cell survival. Phosphorylation at Ser376 is a key post-translational modification linked to the regulation of IKK-γ's activity. Studies suggest that this phosphorylation event may influence NF-κB signaling by modulating IKK-γ's interaction with other proteins or its subcellular localization, particularly during cellular responses to stimuli like cytokines, pathogens, or stress. The IKK-γ (Phospho-Ser376) antibody is widely employed in techniques such as Western blotting, immunoprecipitation, or immunofluorescence to investigate the activation status and functional dynamics of the IKK complex in various contexts, including cancer, autoimmune diseases, and infection. Its specificity for the phosphorylated form makes it valuable for dissecting pathway activation mechanisms and evaluating therapeutic targets aimed at modulating NF-κB activity. Proper validation using phosphorylation-deficient mutants or phosphatase-treated samples is recommended to ensure assay accuracy.