| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ALK tyrosine kinase receptor; anaplastic lymphoma kinase; anaplastic lymphoma kinase (Ki-1); CD246; EC 2.7.10.1; kinase ALK |
| Entrez GeneID | 238; |
| WB Predicted band size | 176kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 1096 (P-N-Y(p)-C-F) derived from Human ALK. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下为3条关于ALK (Phospho-Tyr1096)抗体的参考文献及其摘要概括：
1. **"Activation and signaling by the ALK receptor tyrosine kinase in neuroblastoma"**
- **作者**：Mosse YP et al.
- **摘要**：研究揭示了ALK在神经母细胞瘤中的异常激活机制，利用Phospho-Tyr1096抗体检测其自磷酸化水平，证实Tyr1096是ALK激活的关键位点，并与下游PI3K/AKT信号通路关联。
2. **"Targeted inhibition of ALK tyrosine kinase activity in neuroblastoma"**
- **作者**：George RE et al.
- **摘要**：通过小分子抑制剂阻断ALK活性，使用Phospho-Tyr1096抗体验证抑制剂对ALK磷酸化的抑制效果，为神经母细胞瘤的靶向治疗提供实验依据。
3. **"Structural basis for autoinhibition and ligand-induced activation of ALK"**
- **作者**：Bossi RT et al.
- **摘要**：解析ALK激酶结构，结合Phospho-Tyr1096抗体检测Tyr1096位点的磷酸化状态，阐明其自抑制构象及配体激活后的构象变化，揭示致癌突变对磷酸化的影响。
以上文献均通过Phospho-Tyr1096特异性抗体，探究ALK在肿瘤发生、信号传导及靶向治疗中的作用，涵盖分子机制与临床前研究。
The ALK (Phospho-Tyr1096) antibody is a specialized tool used to detect the activated form of the anaplastic lymphoma kinase (ALK), a receptor tyrosine kinase involved in cell signaling pathways. ALK plays critical roles in nervous system development and is implicated in oncogenesis when aberrantly activated through mutations, gene rearrangements (e.g., EML4-ALK fusion), or overexpression. Phosphorylation at tyrosine 1096 (Y1096) is a key post-translational modification marking ALK activation, typically triggered by ligand binding or constitutive activation in cancer. This phosphorylation event facilitates downstream signaling cascades, including RAS/MAPK, PI3K/AKT, and JAK/STAT pathways, driving cell proliferation, survival, and differentiation.
The ALK (Phospho-Tyr1096) antibody specifically recognizes the phosphorylated Y1096 residue, enabling researchers to study ALK activation status in diseases such as non-small cell lung cancer (NSCLC), neuroblastoma, and anaplastic large cell lymphoma (ALCL). It is widely utilized in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess ALK activity in clinical samples or experimental models. This antibody is particularly valuable for evaluating therapeutic responses to ALK inhibitors (e.g., crizotinib, alectinib) and investigating resistance mechanisms. Its specificity for the phosphorylated epitope makes it essential for distinguishing active ALK from its inactive form, aiding both basic research and translational oncology studies.
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