Rabbit Polyclonal CK-1α(Phospho-Tyr294) Antibody

The CK-1α (Phospho-Tyr294) antibody is a specialized tool used to detect the phosphorylation of Casein Kinase 1 alpha (CK1α) at tyrosine residue 294. CK1α, a member of the serine/threonine protein kinase family, plays critical roles in diverse cellular processes, including Wnt/β-catenin signaling, cell cycle regulation, DNA repair, and circadian rhythms. Its activity is tightly controlled by post-translational modifications, such as phosphorylation, which modulate kinase function, substrate specificity, and interactions with signaling partners. Phosphorylation at Tyr294 is thought to influence CK1α's regulatory capacity, potentially affecting downstream pathways implicated in development and disease. This antibody is specifically designed to recognize the phosphorylated form of Tyr294. enabling researchers to study CK1α activation dynamics in response to stimuli or pathological conditions. It is commonly employed in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate CK1α's role in cancer (e.g., colorectal, breast), neurodegenerative disorders, and metabolic syndromes. Validation typically includes testing in knockout models or phosphorylation-blocking assays to ensure specificity. Understanding CK1α phosphorylation at this site may provide insights into therapeutic targeting, as dysregulated CK1α activity has been linked to tumor progression and other diseases.