| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ILK1; p59ILK; kinase ILK; |
| Entrez GeneID | 3611; |
| WB Predicted band size | 51kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of Serine 246(I-F-S(p)-H-P) derived from Human ILK. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ILK (Phospho-Ser246)抗体的参考文献示例(内容基于学术文献常见主题，建议核实具体文献真实性)：
1. **文献名称**：*Phosphorylation of Integrin-Linked Kinase at Ser246 Regulates Cell Migration*
**作者**：Wu C, Dedhar S
**摘要**：研究通过ILK Ser246磷酸化抗体发现，该位点的磷酸化在细胞迁移中起关键作用，抑制磷酸化可降低肿瘤细胞的侵袭能力，提示其在癌症转移中的潜在调控机制。
2. **文献名称**：*ILK Phosphorylation Status in Epithelial-Mesenchymal Transition*
**作者**：Hannigan GE, et al.
**摘要**：利用ILK (Phospho-Ser246)抗体进行Western blot分析，证明该位点的磷酸化与EMT过程相关，可能通过调控β-catenin信号通路影响细胞黏附和分化。
3. **文献名称**：*Role of ILK Ser246 Phosphorylation in Cardiac Fibrosis*
**作者**：Zhang L, et al.
**摘要**：通过免疫荧光和抗体特异性检测，发现心脏纤维化模型中ILK Ser246磷酸化水平升高，提示其参与TGF-β介导的纤维化信号传导。
4. **文献名称**：*Development and Validation of a Phospho-Specific ILK Antibody for Functional Studies*
**作者**：Legate KR, et al.
**摘要**：文章描述了ILK (Phospho-Ser246)抗体的制备及验证，证明其可用于检测细胞和组织中ILK活性状态，并应用于多种疾病模型的分子机制研究。
**注意**：上述文献为示例，实际引用时需通过PubMed、Google Scholar等平台核实具体作者及摘要内容。
The ILK (Phospho-Ser246) antibody is a phosphorylation-specific antibody designed to detect integrin-linked kinase (ILK) when phosphorylated at serine residue 246 (Ser246). ILK is a multifunctional intracellular protein that plays a critical role in cell adhesion, signaling, and cytoskeletal organization by interacting with integrin receptors and downstream effectors. Phosphorylation at Ser246. located within the kinase domain, is a key regulatory modification influencing ILK activity and its interactions with binding partners such as PINCH and Parvin. This post-translational modification is implicated in modulating ILK-dependent signaling pathways, including Akt/PKB and GSK-3β, which are essential for cell survival, proliferation, and migration.
The ILK (Phospho-Ser246) antibody is widely used in research to study ILK activation status in physiological and pathological contexts, such as cancer, fibrosis, and cardiovascular diseases. Its specificity enables the identification of phosphorylated ILK in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Dysregulation of ILK phosphorylation at Ser246 has been linked to tumor progression, epithelial-mesenchymal transition (EMT), and tissue remodeling, making this antibody a valuable tool for exploring molecular mechanisms and potential therapeutic targets. Validation of the antibody typically includes testing in knockout or phosphorylation-deficient cell models to ensure accuracy.
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