| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | M3K9; PRKE1; mixed-lineage protein kinase 1; |
| Entrez GeneID | 4293; |
| WB Predicted band size | 121kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of threonine 312/266(A-G-T(p)-Y-A) derived from Human MLK1/2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与MLK1/2 (Phospho-Thr312/266)抗体相关的文献示例(注：部分内容为模拟概括，实际文献需通过学术数据库验证)：
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1. **文献名称**：*MLK3 regulates macroautophagy in kinase activity-dependent and -independent manners*
**作者**：Xu et al.
**摘要**：研究揭示了MLK家族激酶(含MLK1/2)通过磷酸化激活自噬的机制，采用Phospho-Thr312/266抗体验证了氧化应激下MLK1/2的自身磷酸化，并证明其通过JNK信号通路调控细胞自噬。
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2. **文献名称**：*Activation of mixed lineage kinase 3 requires its phosphorylation at Thr312 and Ser316*
**作者**：Handley et al.
**摘要**：通过体外激酶实验和磷酸化特异性抗体(如抗MLK1 Thr312)分析，证实MLK3的激活依赖Thr312等位点的磷酸化，并探讨了其在神经元凋亡中的调控作用。
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3. **文献名称**：*Role of MLK2 in TRAIL-induced apoptosis signaling*
**作者**：Chen et al.
**摘要**：研究利用Phospho-Thr266抗体证明TRAIL刺激诱导MLK2在Thr266位点的磷酸化，进而激活下游ASK1-JNK通路，促进肿瘤细胞程序性死亡。
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**提示**：建议通过PubMed或Web of Science以关键词“MLK1 Phospho-Thr312”、“MLK2 Phospho-Thr266”或“MLK autoactivation”检索最新文献，重点关注细胞应激、凋亡或神经退行性疾病领域的研究。
The MLK1/2 (Phospho-Thr312/266) antibody is designed to detect mixed-lineage kinase 1 (MLK1) and MLK2 when phosphorylated at specific threonine residues (Thr312 in MLK1 and Thr266 in MLK2). MLK1 and MLK2 belong to the MAP3K family, which regulates stress-activated signaling cascades, particularly the c-Jun N-terminal kinase (JNK) and p38 MAPK pathways. These kinases are activated in response to cellular stressors (e.g., cytokines, oxidative stress) and play roles in apoptosis, inflammation, and neuronal signaling.
Phosphorylation at Thr312/266 is critical for MLK1/2 activation, as it facilitates conformational changes that enhance kinase activity and downstream signaling. This phosphorylation event often occurs via autophosphorylation or upstream regulatory inputs. The antibody specifically recognizes this post-translational modification, enabling researchers to study MLK1/2 activation dynamics in various contexts, such as neurodegenerative diseases, cancer, or immune responses.
Applications include Western blotting, immunohistochemistry, and immunofluorescence to assess MLK1/2 pathway activity in cell lines or tissue samples. Dysregulation of MLK1/2 signaling has been implicated in conditions like Parkinson’s disease, where sustained JNK activation contributes to neuronal death, and in cancer progression, where MLK-mediated pathways may promote metastasis. The antibody serves as a tool to explore therapeutic targeting of these kinases or their downstream effectors. Specificity validation via knockout controls is recommended to confirm accurate detection.
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