Rabbit Polyclonal MAP3K1(Phospho-Thr1400) Antibody

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     Western blot analysis of extracts from JK cells (Lane 2), using MAP3K1 (Phospho-Thr1400) Antibody P40441. The lane on the left is treated with antigen-specific peptide.    

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     Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K1 (Phospho-Thr1400) antibody P40441 (left)or the same antibody preincubated with blocking peptide (right).    

The MAP3K1 (Phospho-Thr1400) antibody is designed to detect the phosphorylation of mitogen-activated protein kinase kinase kinase 1 (MAP3K1) at threonine residue 1400. MAP3K1. a serine/threonine kinase, plays a pivotal role in regulating multiple signaling pathways, including the JNK, ERK, and NF-κB cascades, which are critical for cellular responses such as proliferation, differentiation, apoptosis, and stress responses. Phosphorylation at Thr1400 is associated with the activation or modulation of MAP3K1 kinase activity, often triggered by extracellular stimuli like cytokines, growth factors, or cellular stress. This post-translational modification serves as a regulatory mechanism, influencing downstream signaling events and crosstalk between pathways.

The antibody is widely used in research to investigate MAP3K1 activation dynamics in physiological and pathological contexts, such as cancer, immune regulation, and developmental processes. It is commonly applied in techniques like Western blotting, immunoprecipitation, and immunohistochemistry to assess phosphorylation-dependent signaling states. Validation typically includes testing in knockout cell lines or samples with known activation status to ensure specificity. Researchers utilize this tool to explore how MAP3K1-mediated signaling contributes to disease mechanisms or therapeutic targets, particularly in oncology, where aberrant MAP3K1 activity is linked to tumor progression and drug resistance. Proper controls are essential to distinguish phosphorylation signals from total protein levels.