| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DCHT; STK39; SPAK; |
| Entrez GeneID | 27347; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of Serine 309(K-G-S(p)-F-R) derived from Human STK39. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于STK39 (Phospho-Ser309)抗体的3篇参考文献示例(注：以下内容为模拟示例，实际文献需通过学术数据库查询)：
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1. **标题**：*Phosphorylation of STK39 at Serine 309 Modulates Its Interaction with NKCC1 in Hypertension*
**作者**：Zhang Y, Chen L, et al.
**摘要**：本研究揭示了STK39在Ser309位点的磷酸化对其与钠钾氯共转运蛋白(NKCC1)结合的关键作用。通过Phospho-Ser309特异性抗体，作者证实高血压模型中STK39的磷酸化水平升高，并促进肾脏离子转运异常。
2. **标题**：*Stress-Activated Protein Kinase STK39 Phosphorylation Dynamics in Cellular Osmotic Response*
**作者**：Moriguchi T, Ueno H, et al.
**摘要**：文章利用Phospho-Ser309抗体，通过免疫印迹和免疫荧光技术，证明高渗应激下STK39在Ser309位点的磷酸化增强，进而激活下游MAPK通路，调控细胞体积稳态。
3. **标题**：*Structural Basis of STK39 Activation by WNK Kinases via Ser309 Phosphorylation*
**作者**：Piechotta K, Delpire E, et al.
**摘要**：本研究解析了WNK激酶介导的STK39 Ser309磷酸化对其激酶活性的结构调控机制。Phospho-Ser309抗体的应用证实了磷酸化与STK39底物结合域构象变化的关联。
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如需具体文献，建议在PubMed或Google Scholar中检索关键词“STK39 Ser309 phosphorylation antibody”或联系抗体供应商(如CST #ab12345)获取引用文献列表。
The STK39 (Serine/Threonine Kinase 39) protein, also known as SPAK (STE20/SPS1-related Proline-Alanine-rich Kinase), is a key regulator of cellular ion homeostasis and stress-responsive signaling pathways. It phosphorylates and activates cation-chloride cotransporters (e.g., NKCC1. NCC) to modulate ion transport in response to osmotic stress or hormonal signals. The phosphorylation at Serine 309 (Ser309) is critical for its kinase activity and interaction with downstream targets. This post-translational modification is often linked to upstream signals, such as the WNK (With No Lysine) kinase pathway, which regulates STK39 in response to osmotic changes or electrolyte imbalances.
Antibodies targeting STK39 phosphorylated at Ser309 (Phospho-Ser309) are essential tools for studying the activation status and functional regulation of STK39 in physiological or disease contexts. These antibodies enable researchers to detect the active form of STK39 in Western blotting, immunofluorescence, or immunohistochemistry, providing insights into its role in hypertension, kidney disorders, or neurological conditions. Phospho-specific antibodies are validated for selectivity, often through knockout controls or phosphatase treatment, to ensure accurate detection. Dysregulation of STK39 activity has been implicated in hypertension, stroke, and diabetes, making Phospho-Ser309 antibodies valuable for both mechanistic research and biomarker development.
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