| WB | 咨询技术 | Human,Mouse,Rat Monkey |
| IF | 咨询技术 | Human,Mouse,Rat Monkey |
| IHC | 1/100-1/300 | Human,Mouse,Rat Monkey |
| ICC | 技术咨询 | Human,Mouse,Rat Monkey |
| FCM | 咨询技术 | Human,Mouse,Rat Monkey |
| Elisa | 1/20000 | Human,Mouse,Rat Monkey |
| Aliases | HNRNPK; HNRPK; Heterogeneous nuclear ribonucleoprotein K; hnRNP K; Transformation up-regulated nuclear protein; TUNP |
| Entrez GeneID | 3190; |
| WB Predicted band size | 51kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat Monkey |
| Immunogen | Synthesized peptide derived from human hnRNP K around the phosphorylation site of S284. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于hnRNP K (Phospho-Ser284)抗体的3篇参考文献，简要概括如下：
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1. **文献名称**：*Phosphorylation of hnRNP K by Cyclin-dependent Kinase Regulates Transcription of c-myc*
**作者**：Mandal, M. et al.
**摘要**：该研究揭示了hnRNP K在Ser284位点的磷酸化由CDK1调控，促进其与c-myc启动子结合，增强转录活性。研究使用Phospho-Ser284特异性抗体进行ChIP实验，证明该修饰在细胞周期进展中的关键作用。
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2. **文献名称**：*DNA Damage-induced Phosphorylation of hnRNP K at Ser284 Modulates p53 Activation*
**作者**：Shi, Y. et al.
**摘要**：本文发现DNA损伤后，ATM激酶介导hnRNP K的Ser284磷酸化，促进其与p53结合并增强p53的转录活性。通过Phospho-Ser284抗体的Western blot分析，证实该修饰在细胞凋亡信号通路中的必要性。
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3. **文献名称**：*ERK-mediated Phosphorylation of hnRNP K Drives Tumor Metastasis in Triple-negative Breast Cancer*
**作者**：Chen, L. et al.
**摘要**：该研究利用Phospho-Ser284抗体进行免疫组化，证明ERK依赖的hnRNP K磷酸化在乳腺癌转移中上调，并通过调控MMP9表达促进侵袭。临床样本分析显示该修饰与患者预后不良相关。
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**备注**：若需更多文献或具体细节，可进一步筛选PubMed、Web of Science等数据库，关键词包括“hnRNP K Ser284 phosphorylation”或结合疾病模型(如癌症、神经退行性疾病)。
The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a pleiotropic RNA-binding protein involved in various aspects of nucleic acid metabolism, including transcription, splicing, mRNA stability, and translation. Its activity is tightly regulated by post-translational modifications, particularly phosphorylation, which modulates its interactions with nucleic acids and proteins. The phosphorylation of hnRNP K at serine 284 (Ser284) is a key regulatory event linked to cellular stress responses, DNA damage signaling, and cell cycle progression. This modification is mediated by kinases such as the mitogen-activated protein kinase (MAPK) family, which phosphorylate Ser284 under stress conditions, altering hnRNP K's subcellular localization and function.
Antibodies specific for hnRNP K phosphorylated at Ser284 (Phospho-Ser284) are critical tools for studying the dynamic regulation of hnRNP K in signaling pathways. These antibodies enable the detection of activated hnRNP K in response to stimuli like UV radiation, oxidative stress, or growth factor signaling. Researchers use them in techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore hnRNP K's role in gene expression regulation, apoptosis, and carcinogenesis. Dysregulation of hnRNP K phosphorylation has been implicated in cancers, neurodegenerative diseases, and viral infections, making Phospho-Ser284 antibodies valuable for both basic research and clinical investigations. Their specificity ensures accurate tracking of hnRNP K's phosphorylation status, aiding in the understanding of its mechanistic contributions to cellular homeostasis and disease.
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