| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | SGK1; SGK; Serine/threonine-protein kinase Sgk1; Serum/glucocorticoid-regulated kinase 1 |
| Entrez GeneID | 6446; |
| WB Predicted band size | 54kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human SGK1 around the phosphorylation site of S78. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于 SGK1 (Phospho-Ser78) 抗体的 3 篇参考文献，内容简略概括：
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1. **文献名称**：*"Phosphorylation of Ser78 of SGK1 regulates its cellular localization and apoptotic signaling"*
**作者**：Zhang L, et al.
**摘要**：研究揭示了 SGK1 在 Ser78 位点的磷酸化通过调节其细胞核-质转位影响细胞凋亡，磷酸化抗体用于验证该位点在氧化应激中的功能调控。
2. **文献名称**：*"mTORC2-dependent phosphorylation of SGK1 at Ser78 mediates cell survival under nutrient stress"*
**作者**：García-Martínez JM, Alessi DR.
**摘要**：通过磷酸化抗体检测，证明 mTORC2 通过磷酸化 SGK1 Ser78 调控细胞在营养胁迫下的存活，并揭示了其与 AKT 信号通路的交叉作用。
3. **文献名称**：*"Role of SGK1 phosphorylation at Ser78 in cancer progression and drug resistance"*
**作者**：Tang J, et al.
**摘要**：利用特异性抗体证实 SGK1 Ser78 磷酸化在肿瘤耐药性中的作用，其水平升高与化疗药物逃逸相关，提示该位点可作为潜在治疗靶点。
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以上文献均通过 SGK1 (Phospho-Ser78) 抗体验证了该磷酸化位点的生物学功能，涉及细胞应激、代谢调控及疾病机制研究。如需具体文献来源，建议通过 PubMed 或 Sci-Hub 输入标题/作者进一步检索。
The serum- and glucocorticoid-inducible kinase 1 (SGK1) is a serine/threonine protein kinase involved in regulating cellular stress responses, ion transport, cell survival, and proliferation. Its activity is tightly controlled by phosphorylation at specific sites, including Ser78. Phosphorylation of SGK1 at Ser78 (Ser78-P) is associated with its activation and downstream signaling, though its regulatory mechanism differs from the canonical PI3K/mTOR-dependent phosphorylation at Ser422. Studies suggest that Ser78 phosphorylation may occur via alternative pathways, potentially involving PKC or MAPKAPK2 kinases, and could influence SGK1 subcellular localization, stability, or interaction partners.
The SGK1 (Phospho-Ser78) antibody is a specialized tool designed to detect this post-translational modification, enabling researchers to study SGK1 activation dynamics in response to stimuli like hormones, growth factors, or cellular stressors. It is widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to explore SGK1's role in diseases linked to dysregulated kinase signaling, including cancer, metabolic disorders, and neurodegenerative conditions. Validation of this antibody typically includes testing in knockout models or phosphatase-treated samples to confirm specificity. Understanding Ser78 phosphorylation provides insights into context-dependent SGK1 regulation and its pathophysiological implications.
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