Rabbit Polyclonal Csk(Phospho-Ser364) Antibody

The Csk (Phospho-Ser364) antibody is a specific immunological tool designed to detect the phosphorylation of C-terminal Src kinase (Csk) at serine residue 364. Csk is a key regulatory kinase that phosphorylates the inhibitory tyrosine residue of Src-family kinases (SFKs), such as Src, Fyn, and Lyn, thereby suppressing their activity. This negative regulation is critical for maintaining cellular homeostasis, as uncontrolled SFK activity is linked to aberrant cell proliferation, migration, and oncogenesis. Phosphorylation at Ser364 in Csk has been implicated in modulating its own kinase activity, subcellular localization, or interactions with binding partners, though the precise functional consequences remain under investigation. This post-translational modification may serve as a regulatory mechanism to fine-tune Csk-mediated SFK inhibition in response to extracellular signals or cellular stress. The Csk (Phospho-Ser364) antibody enables researchers to study the activation status and regulatory pathways of Csk in diverse biological contexts, including cancer, immune signaling, and neuronal development. It is commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to analyze phosphorylation-dependent Csk dynamics in cell lines, tissues, or disease models. Validation typically includes testing in phosphorylation-deficient mutants or phosphatase-treated samples to confirm specificity.