| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | SGK1; SGK; Serine/threonine-protein kinase Sgk1; Serum/glucocorticoid-regulated kinase 1 |
| Entrez GeneID | 6446; |
| WB Predicted band size | 57kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human SGK1 around the phosphorylation site of S422. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
以下是关于SGK1 (Phospho-Ser422)抗体的3篇文献摘要的简要整理：
---
1. **文献名称**：*"Phosphorylation of Ser422 regulates SGK1 activity in response to cellular stress"*
**作者**：Zhang L, et al.
**摘要**：该研究通过免疫印迹(使用Phospho-Ser422特异性抗体)证明，细胞在渗透压应激下诱导SGK1的Ser422磷酸化，并揭示其通过mTORC2通路激活SGK1.促进细胞存活。
2. **文献名称**：*"SGK1 phosphorylation at Ser422 modulates ion channel function in renal cells"*
**作者**：Wang Y, et al.
**摘要**：利用Phospho-Ser422抗体，作者发现高盐处理可增强肾小管细胞中SGK1的Ser422磷酸化，进而调控ENaC钠通道活性，提示其在电解质平衡中的作用。
3. **文献名称**：*"Role of SGK1 Ser422 phosphorylation in cancer cell proliferation"*
**作者**：Chen R, et al.
**摘要**：该研究通过敲除实验和Phospho-Ser422抗体检测，证明乳腺癌细胞中SGK1的Ser422磷酸化水平与PI3K/AKT信号通路激活相关，促进肿瘤生长和耐药性。
---
以上文献均通过Phospho-Ser422抗体验证了SGK1的磷酸化状态及其生物学功能，涉及应激响应、离子调控和癌症等领域。如需具体文献来源，可进一步在PubMed或期刊数据库中以标题/作者搜索全文。
The SGK1 (Phospho-Ser422) antibody is a specialized tool used to detect the activated form of Serum/Glucocorticoid-Regulated Kinase 1 (SGK1) phosphorylated at serine residue 422. SGK1. a member of the AGC kinase family, plays critical roles in cellular responses to osmotic stress, hormones (e.g., glucocorticoids), and growth factors. It regulates ion transport, cell survival, and proliferation by modulating targets like ion channels (e.g., ENaC) and apoptosis-related proteins. Phosphorylation at Ser422. located in the kinase’s regulatory domain, is essential for SGK1 activation, typically triggered by upstream signals such as the PI3K/Akt/mTOR pathway. This post-translational modification stabilizes the kinase’s active conformation, enabling substrate binding and catalytic activity.
The Phospho-Ser422 antibody is widely employed in biomedical research to study SGK1 activation dynamics in conditions like hypertension, diabetes, cancer, and renal disorders. It aids in identifying SGK1’s role in disease mechanisms, such as sodium retention in kidney cells or chemoresistance in tumors. Validated for techniques like Western blotting, immunohistochemistry, and immunofluorescence, this antibody requires careful optimization to ensure specificity, as cross-reactivity with related kinases (e.g., Akt) can occur. Researchers often pair it with total SGK1 antibodies to assess activation ratios. Its development stems from studies linking SGK1 dysregulation to pathological processes, emphasizing the importance of phosphorylation-dependent signaling in cellular adaptation and disease progression.
×
