Rabbit Polyclonal Shb(Phospho-Tyr246) Antibody

The Shb (Phospho-Tyr246) antibody is designed to detect the adaptor protein Shb (SH2 domain-containing adaptor protein B) when phosphorylated at tyrosine residue 246. Shb is a signaling protein involved in regulating cellular processes such as proliferation, differentiation, and apoptosis. It contains multiple functional domains, including an N-terminal phosphotyrosine-binding (PTB) domain, a central proline-rich region, and a C-terminal SH2 domain, enabling interactions with various signaling molecules like receptor tyrosine kinases (RTKs), integrins, and cytosolic kinases. Phosphorylation at Tyr246 is a critical post-translational modification that modulates Shb’s ability to recruit downstream effectors, influencing pathways such as PI3K/AKT, MAPK/ERK, and PLCγ. This phosphorylation event often occurs in response to extracellular stimuli, including growth factors or cytokines, and is implicated in angiogenesis, immune responses, and cancer progression. The Shb (Phospho-Tyr246) antibody is widely used in research to study Shb’s role in signal transduction, particularly in contexts like tumorigenesis, diabetes, or vascular development. Specificity is typically validated via knockout controls or peptide competition assays. Applications include Western blotting, immunoprecipitation, and immunohistochemistry to assess Shb activation status in cell lines, tissues, or disease models, providing insights into its mechanistic contributions to cellular signaling networks.