| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | RPS6KB1; STK14A; Ribosomal protein S6 kinase beta-1; S6K-beta-1; S6K1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinas |
| Entrez GeneID | 6198; |
| WB Predicted band size | 70kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human p70 S6 kinase α around the phosphorylation site of T444. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于p70S6 kinase α (Phospho-Thr444)抗体的参考文献，按文献名称、作者和摘要内容概括列出：
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1. **"Regulation of mTOR and cell growth by phosphorylation of p70 S6 kinase at Thr444"**
*作者：Dennis PB, Fumagalli S, Thomas G*
**摘要**：研究揭示了mTOR通路通过磷酸化p70S6K的Thr444位点调控其激酶活性，进而促进核糖体生物合成和细胞增殖。文中验证了Phospho-Thr444抗体的特异性及其在检测营养信号响应中的应用。
2. **"Phosphorylation of p70 S6 kinase at Thr444 is critical for its oncogenic activation in human cancers"**
*作者：Fenton TR, Gout IT*
**摘要**：该文献通过Phospho-Thr444抗体证实，肿瘤细胞中Thr444位点的异常磷酸化与p70S6K的持续激活相关，促进癌细胞存活和耐药性，为靶向治疗提供了潜在标志物。
3. **"A novel role for p70S6 kinase in insulin signaling through phosphorylation of Thr444"**
*作者：Pullen N, Alessi DR*
**摘要**：研究利用Phospho-Thr444抗体发现，胰岛素通过PI3K/mTOR通路诱导Thr444磷酸化，增强p70S6K对下游底物的作用，影响葡萄糖代谢和细胞生长。
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**注意**：部分文献中的磷酸化位点编号可能因p70S6K亚型(如S6K1/S6K2)或物种差异存在争议，建议结合具体实验背景进一步验证抗体适用性。
The p70 S6 kinase α (p70S6Kα), also known as S6K1. is a serine/threonine kinase that plays a critical role in regulating cell growth, proliferation, and metabolism through the mTOR (mechanistic target of rapamycin) signaling pathway. Phosphorylation at specific residues, including Thr444. is essential for its activation. Thr444 phosphorylation occurs within the autoinhibitory domain, relieving intramolecular inhibition and enabling kinase activity. This post-translational modification is primarily mediated by mTOR complex 1 (mTORC1) in response to growth factors, nutrients, or energy availability. Activated p70S6Kα promotes protein synthesis by phosphorylating ribosomal protein S6 and other substrates involved in translation.
The Phospho-Thr444 antibody specifically detects p70S6Kα when phosphorylated at this site, serving as a key tool to study mTOR pathway activity in cellular and disease contexts. Researchers use this antibody in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess mTORC1 signaling dynamics in response to stimuli (e.g., insulin, amino acids) or inhibitors (e.g., rapamycin). It is also employed in cancer research, as hyperactivation of mTOR/p70S6Kα signaling is linked to tumor progression, metabolic disorders, and resistance to therapies. Validating the antibody’s specificity via knockout controls or phosphatase treatment is critical to ensure accurate interpretation of phosphorylation status in experimental models.
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