Rabbit Polyclonal NF2(Phospho-Ser518) Antibody

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     Western blot analysis of HELA using p-NF2 (S518) antibody.    

The NF2 (Phospho-Ser518) antibody is a specialized tool used to detect the phosphorylated form of the neurofibromin 2 (NF2) protein at serine residue 518. The NF2 gene encodes merlin (moesin-ezrin-radixin-like protein), a tumor suppressor that regulates cell proliferation, adhesion, and cytoskeletal organization. Merlin's activity is modulated by phosphorylation, particularly at Ser518. which alters its conformation and function. Phosphorylation at this site, mediated by kinases such as PAK1 and PKA, inactivates merlin by maintaining it in an "open" state, disrupting its ability to suppress tumor growth. Conversely, dephosphorylation by phosphatases like myosin phosphatase restores its "closed," active conformation.

This antibody is crucial for studying merlin's regulatory mechanisms in diseases linked to NF2 mutations, such as neurofibromatosis type 2 (characterized by benign nervous system tumors) and sporadic cancers like mesothelioma. Researchers employ it in techniques like Western blotting, immunohistochemistry, or immunofluorescence to assess phosphorylation status in cell lines or tissue samples. By detecting p-Ser518 merlin, the antibody helps elucidate pathways involving merlin inactivation, Hippo signaling dysregulation, or therapeutic responses in preclinical models. Its specificity for the phosphorylated epitope makes it valuable for distinguishing active/inactive merlin states in both basic research and translational studies.