Rabbit Polyclonal Pragmin(Phospho-Tyr413) Antibody

The Pragmin (Phospho-Tyr413) antibody is designed to detect Pragmin (also known as SgK223) when phosphorylated at tyrosine residue 413 (Y413). Pragmin is a scaffolding protein belonging to the pseudokinase family, lacking catalytic activity but playing a role in cellular signaling. It interacts with protein phosphatases and kinases, regulating pathways involved in cell adhesion, migration, and polarity. Phosphorylation at Y413. mediated by Src-family kinases (SFKs), is critical for Pragmin’s function in promoting oncogenic signaling and metastatic behavior in cancer cells. This modification enhances Pragmin’s ability to recruit downstream effectors, such as the protein tyrosine phosphatase PTPRT, facilitating signaling crosstalk linked to cytoskeletal reorganization and cell invasion. The Phospho-Tyr413 antibody is widely used in cancer research to study Pragmin’s activation status and its role in tumor progression, particularly in contexts like epithelial-mesenchymal transition (EMT) and metastasis. It is validated for applications including Western blotting, immunoprecipitation, and immunofluorescence, helping researchers assess phosphorylation-dependent interactions and pathway activation. Specificity is confirmed using phosphorylation-blocking peptides or cells treated with SFK inhibitors. Proper storage (in aliquots at -20°C) and optimized dilution protocols are recommended to maintain antibody performance.