Rabbit Polyclonal p7S6kinaseα(Phospho-Ser447) Antibody

中文名： p7S6 kinase α (Phospho-Ser447)抗体
别名： RPS6KB1; STK14A; Ribosomal protein S6 kinase beta-1; S6K-beta-1; S6K1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinas

货号: IPDX40810

Price： ￥1280

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	咨询技术	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	1/100-1/300	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	咨询技术	Human,Mouse,Rat
Elisa	1/10000	Human,Mouse,Rat

产品详情

Aliases	RPS6KB1; STK14A; Ribosomal protein S6 kinase beta-1; S6K-beta-1; S6K1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinas
Entrez GeneID	6198;
WB Predicted band size	70kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human p70 S6 kinase α around the phosphorylation site of S447.
Formulation	Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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Western Blot analysis of A549 cells using Phospho-p70 S6 kinase α (S447) Polyclonal Antibody

参考文献

以下是3篇关于p70S6 kinase α (Phospho-Ser447)抗体的参考文献示例(注：部分文献信息为模拟概括，实际引用需核实)：

1. **"mTOR-dependent phosphorylation of p70S6 kinase α at Ser447 regulates cellular growth"**

*Authors: Smith J, et al.*

**摘要**：本研究利用Phospho-Ser447特异性抗体，揭示了mTORC1通过磷酸化p70S6Kα的Ser447位点调控细胞增殖的机制，并验证该抗体在肿瘤细胞系中的检测特异性。

2. **"Development of a phosphospecific antibody for p70S6Kα (Ser447) and its application in metabolic studies"**

*Authors: Lee H, et al.*

**摘要**：作者开发了一种针对Ser447磷酸化位点的单克隆抗体，通过免疫印迹和免疫荧光验证其灵敏度，并应用于高糖条件下胰岛β细胞中p70S6Kα激活途径的研究。

3. **"Ser447 phosphorylation of p70S6Kα mediates feedback inhibition by ribosomal protein S6"**

*Authors: Chen R, et al.*

**摘要**：使用Phospho-Ser447抗体证实，核糖体蛋白S6通过负反馈调节p70S6Kα的Ser447磷酸化，影响mTOR信号通路的动态平衡，为癌症靶向治疗提供新依据。

**注意**：以上文献为示例性质，具体研究需根据实际数据库(如PubMed、Google Scholar)检索确认。若目标位点或抗体名称存在差异，建议结合“p70S6K”、“phosphorylation site”及“antibody validation”等关键词进一步筛选。

背景信息

The p70S6 kinase α (Phospho-Ser447) antibody is a specialized tool used to detect the phosphorylated form of p70S6 kinase α (also known as S6K1) at serine residue 447. p70S6 kinase α, a member of the AGC kinase family, plays a critical role in regulating cell growth, proliferation, and metabolism by integrating signals from the mTORC1 (mechanistic target of rapamycin complex 1) pathway. Phosphorylation at Ser447 is associated with the enzyme's activation, which occurs downstream of growth factors, hormones, or nutrient availability. This phosphorylation event is part of a complex regulatory mechanism involving multiple phosphorylation sites, including Thr389. which is essential for full kinase activity.

The antibody is widely used in research to study mTORC1 signaling dynamics, particularly in contexts such as cancer, metabolic disorders, and aging. By specifically recognizing the Ser447-phosphorylated state, it allows researchers to assess pathway activation in various experimental models, including cell cultures, tissues, or animal samples. Applications include Western blotting, immunofluorescence, and immunohistochemistry. Validation of this antibody typically involves testing in knockout or phosphatase-treated controls to confirm specificity. Its use has contributed to understanding dysregulated mTOR signaling in diseases and evaluating therapeutic agents targeting this pathway.