| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MAP2K1; MEK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1 |
| Entrez GeneID | 5604; |
| WB Predicted band size | 45kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human MEK-1 around the phosphorylation site of S298. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于 **MEK-1 (Phospho-Ser298) 抗体** 的3篇参考文献示例(文献信息为模拟，仅供参考)：
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1. **文献名称**：*"Phosphorylation of MEK1 at Ser298 regulates its interaction with RAF kinases and cell proliferation"*
**作者**：Catalanotti, F., Reyes, G., & Cobb, M.H.
**摘要**：研究通过突变分析和免疫印迹(使用Phospho-Ser298抗体)发现，MEK1的Ser298磷酸化是RAF激酶激活MEK1的关键步骤，影响细胞增殖和ERK信号通路的正向调控。
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2. **文献名称**：*"MEK1 phosphorylation at Ser298: A biomarker for resistance to BRAF inhibitors in melanoma"*
**作者**：Smalley, K.S., Xiao, Y., & Herlyn, M.
**摘要**：该研究利用MEK1 (Phospho-Ser298)特异性抗体，在黑色素瘤细胞中发现Ser298的高磷酸化水平与BRAF抑制剂耐药性相关，提示其可作为治疗靶点。
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3. **文献名称**：*"A regulatory mechanism controlling MEK1 phosphorylation by upstream kinases"*
**作者**：Robinson, M.J., Cheng, M., & Khokhlatchev, A.V.
**摘要**：通过体外激酶实验和Western blot(使用Phospho-Ser298抗体)，证实MEK1的Ser298磷酸化由PAK激酶介导，并增强其与RAF的结合能力。
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4. **文献名称**：*"Site-specific antibodies reveal distinct phosphorylation states of MEK1 in vivo"*
**作者**：Eblen, S.T., Catling, A.D., & Weber, M.J.
**摘要**：通过开发位点特异性抗体(包括Phospho-Ser298)，研究揭示了MEK1在不同细胞刺激下的动态磷酸化模式及其在信号转导中的功能差异。
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(注：以上文献及作者为示例性内容，实际引用时请核实真实文献信息。)
The MEK-1 (Phospho-Ser298) antibody is a specialized tool used to study the phosphorylation status of MEK-1 (MAPK/ERK kinase 1) at serine residue 298. a critical post-translational modification involved in regulating its activity. MEK-1. a dual-specificity kinase in the MAPK/ERK signaling pathway, acts downstream of Raf kinases to phosphorylate and activate ERK1/2. influencing cell proliferation, differentiation, and survival. Phosphorylation at Ser298. located in the kinase domain, is associated with MEK-1 activation and its interaction with scaffolding proteins or upstream regulators like Raf. This modification may enhance MEK-1 stability, subcellular localization, or signaling efficiency.
The antibody is widely utilized in biomedical research to investigate MEK-1 activation dynamics in response to growth factors, stress stimuli, or oncogenic mutations. It helps detect phosphorylation changes in diseases like cancer, where aberrant MAPK/ERK signaling is common, or in studies exploring targeted therapies (e.g., MEK inhibitors). Validated for techniques like Western blotting, immunoprecipitation, or immunofluorescence, it typically demonstrates specificity for the phosphorylated Ser298 epitope while not cross-reacting with non-phosphorylated MEK-1 or other phospho-serine residues. Proper controls (e.g., phosphatase treatment or knockout samples) are recommended to confirm signal specificity. Its application contributes to understanding MEK-1 regulatory mechanisms and their implications in cellular physiology and pathology.
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