| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | PRKD2; PKD2; HSPC187; Serine/threonine-protein kinase D2; nPKC-D2 |
| Entrez GeneID | 25865; |
| WB Predicted band size | 96kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human PKD2 around the phosphorylation site of S876. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
以下为基于知识背景模拟生成的参考文献示例(请注意这些为虚构内容，建议通过学术数据库核实真实文献)：
1. **文献名称**: "Phosphorylation of PKD2 at Ser876 promotes cell migration in breast cancer"
**作者**: Li et al.
**摘要**: 研究揭示了PKD2在Ser876位点的磷酸化通过激活Akt通路促进乳腺癌细胞迁移。作者使用Phospho-Ser876特异性抗体进行Western blot和免疫荧光实验，证实该位点磷酸化水平与肿瘤转移正相关。
2. **文献名称**: "Regulation of cardiac hypertrophy by PKD2 phosphorylation at Ser876"
**作者**: Martinez et al.
**摘要**: 通过构建Ser876突变小鼠模型，结合Phospho-Ser876抗体检测，发现压力超负荷诱导的PKD2磷酸化通过调控NFAT信号通路驱动心肌细胞肥大，该抗体被用于心肌组织免疫组化分析。
3. **文献名称**: "PKD2 Ser876 phosphorylation mediates oxidative stress-induced apoptosis"
**作者**: Kim & Park
**摘要**: 研究报道H2O2刺激下PKD2在Ser876位点的磷酸化通过激活JNK通路诱导细胞凋亡。使用Phospho-Ser876抗体进行免疫沉淀及激酶活性检测，证实该修饰对凋亡信号传导的关键作用。
4. **文献名称**: "Development and validation of a phosphospecific antibody targeting PKD2 Ser876"
**作者**: Smith et al.
**摘要**: 该文献详细描述了Phospho-Ser876抗体的制备与验证过程，包括肽段免疫、抗体特异性测试(如点杂交和磷酸酶处理对照)，并展示其在流式细胞术中的应用案例。
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**建议检索方法**：
在PubMed或Google Scholar中使用以下关键词组合：
```sql
"PKD2" AND "Ser876" AND (phosphorylation OR antibody)
"Protein Kinase D2" AND "phospho-Ser876"
```
可筛选出涉及该位点功能研究或抗体开发的相关文献。
The PKD2 (Phospho-Ser876) antibody is designed to detect endogenous levels of protein kinase D2 (PKD2) when phosphorylated at serine 876 (Ser876), a key post-translational modification regulating its activity. PKD2. a member of the calcium/calmodulin-dependent kinase superfamily, plays critical roles in cellular processes such as proliferation, apoptosis, and vesicle trafficking. Phosphorylation at Ser876. located within the activation loop of the kinase domain, is associated with PKD2 activation. This modification typically occurs downstream of signaling pathways involving G protein-coupled receptors (GPCRs) or growth factor receptors, often mediated by protein kinase C (PKC) isoforms. The phosphorylation event triggers conformational changes, enabling PKD2 to phosphorylate downstream targets, including substrates involved in stress responses and transcriptional regulation.
The PKD2 (Phospho-Ser876) antibody is widely used in research to study PKD2 activation dynamics in contexts such as cancer, cardiovascular diseases, and immune regulation. It is validated for applications like Western blotting, immunofluorescence, and immunoprecipitation, providing specificity for the phosphorylated form over non-phosphorylated PKD2. Studies utilizing this antibody have elucidated PKD2's role in tumor cell invasion, angiogenesis, and chemoresistance, particularly in cancers like prostate and pancreatic malignancies. Its ability to distinguish active PKD2 makes it a valuable tool for investigating signaling crosstalk and therapeutic targeting in disease models.
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