| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | KIF11; EG5; KNSL1; TRIP5; Kinesin-like protein KIF11; Kinesin-like protein 1; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5 |
| Entrez GeneID | 3832; |
| WB Predicted band size | 119kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from human Eg5 around the phosphorylation site of T926. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Eg5 (Phospho-Thr926)抗体的3篇文献示例(内容为模拟，仅供参考)：
1. **文献名称**：*Phosphorylation of Eg5 at Thr926 regulates spindle assembly in human cells*
**作者**：Smith A, et al.
**摘要**：研究揭示了Eg5在Thr926位点的磷酸化对有丝分裂纺锤体形成的调控作用，使用Phospho-Thr926抗体通过免疫印迹和免疫荧光验证了该修饰在细胞周期中的动态变化。
2. **文献名称**：*A novel role of Eg5 phosphorylation in cancer cell proliferation*
**作者**：Chen L, et al.
**摘要**：通过Phospho-Thr926抗体发现，Eg5的Thr926磷酸化水平在多种癌细胞系中显著升高，抑制该位点磷酸化可阻滞肿瘤细胞分裂，提示其作为潜在治疗靶点。
3. **文献名称**：*Monastrol-induced conformational changes enhance Eg5 phosphorylation at Thr926*
**作者**：Wang X, et al.
**摘要**：利用Phospho-Thr926抗体证实，Eg5抑制剂Monastrol通过诱导构象变化促进Thr926位点磷酸化，进而影响其与微管的结合能力，为药物机制研究提供依据。
The Eg5 (Phospho-Thr926) antibody is a specialized tool used to detect the phosphorylation status of the kinesin-like protein Eg5 (KIF11) at threonine 926. a critical post-translational modification involved in mitotic regulation. Eg5. a member of the kinesin-5 family, plays an essential role in forming and maintaining the bipolar mitotic spindle by generating antiparallel microtubule sliding forces during mitosis. Its activity is tightly regulated through phosphorylation at specific residues, including Thr926. which is believed to modulate Eg5’s motor function, localization, or interactions with other mitotic components. Phosphorylation at this site is associated with cell cycle progression, particularly during metaphase, and may be mediated by kinases such as CDK1 or Plk1.
The Eg5 (Phospho-Thr926) antibody is widely utilized in research to investigate mitotic mechanisms, spindle dynamics, and cell cycle checkpoints. It enables the detection of Eg5 activation states in techniques like Western blotting, immunofluorescence, or immunohistochemistry. Dysregulation of Eg5 phosphorylation has been linked to mitotic defects, chromosomal instability, and diseases such as cancer, where Eg5 overexpression is common. This antibody also serves as a valuable tool in evaluating the efficacy of Eg5-targeted inhibitors, which are explored as potential anticancer therapies due to their ability to induce mitotic arrest. Researchers rely on this antibody to dissect Eg5’s functional roles in both normal and pathological cellular contexts.
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