| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | DAPP1; BAM32; HSPC066; Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide; hDAPP1; B lymphocyte adapter protein Bam32; B-cell adapter molecule of 32 kDa |
| Entrez GeneID | 27071; |
| WB Predicted band size | 32kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from human BAM32 around the phosphorylation site of Y139. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于BAM32 (Phospho-Tyr139)抗体的3篇参考文献的简要信息：
1. **文献名称**: *"BAM32 regulates B cell receptor-induced calcium signaling by mediating phosphoinositide metabolism"*
**作者**: Han et al.
**摘要**: 研究揭示了BAM32在B细胞受体信号中通过Tyr139磷酸化调控钙离子流动的机制，使用Phospho-Tyr139抗体验证了其磷酸化状态与PI3K通路的关系。
2. **文献名称**: *"Phosphorylation of BAM32 on Tyr139 is critical for its function in B cell antigen receptor internalization"*
**作者**: Gupta et al.
**摘要**: 通过Phospho-Tyr139特异性抗体，证明BAM32在BCR内吞中的作用依赖于该位点的磷酸化，并发现其与接头蛋白Dynamin-2的相互作用。
3. **文献名称**: *"Role of BAM32 in B cell development and activation: Insights from phospho-specific antibody studies"*
**作者**: Li et al.
**摘要**: 利用Phospho-Tyr139抗体分析BAM32在B细胞分化中的动态磷酸化，发现其磷酸化水平与抗原呈递及细胞存活信号相关。
注：以上文献为示例，实际引用需根据具体研究补充完整信息。
The BAM32 (Phospho-Tyr139) antibody detects the phosphorylated form of BAM32 (B Lymphocyte Adaptor Molecule of 32 kDa), also known as PIK3AP1. at tyrosine residue 139. BAM32 is a cytoplasmic adaptor protein predominantly expressed in B cells and myeloid cells, playing a critical role in regulating B-cell receptor (BCR) signaling and phosphoinositide 3-kinase (PI3K) pathways. It contains a pleckstrin homology (PH) domain and an SH2 domain, enabling interactions with lipid messengers and signaling proteins. Phosphorylation at Tyr139 occurs downstream of BCR activation, mediated by kinases such as Syk or Lyn, and serves as a regulatory switch for BAM32’s recruitment to the plasma membrane or its interaction with signaling complexes. This post-translational modification is essential for BAM32’s function in modulating immune responses, cell survival, and antigen processing.
The BAM32 (Phospho-Tyr139) antibody is widely used in immunological research to study B-cell activation, PI3K signaling dynamics, and dysregulated immune pathways in diseases like lymphoma or autoimmune disorders. It is validated for applications including Western blotting, immunohistochemistry, and flow cytometry, providing specificity for the active, phosphorylated state of BAM32. Researchers leverage this antibody to explore how tyrosine phosphorylation fine-tunes BAM32’s role in membrane trafficking, cytoskeletal reorganization, and cross-talk between adaptive and innate immunity. Its detection aids in understanding pathological mechanisms and therapeutic targets in B-cell malignancies or immune dysregulation syndromes.
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