| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | KRT18; CYK18; PIG46; Keratin; type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18 |
| Entrez GeneID | 3875; |
| WB Predicted band size | 48kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from human Cytokeratin 18 around the phosphorylation site of S52. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Cytokeratin 18 (Phospho-Ser52)抗体的3篇参考文献及其摘要概述：
1. **文献名称**: *Caspase-mediated cleavage of cytokeratin 18 and histone deubiquitination in apoptosis*
**作者**: Leers, M.P.G. et al.
**摘要**: 研究利用抗Cytokeratin 18 (Phospho-Ser52)的抗体(如M30抗体)检测细胞凋亡过程中CK18的磷酸化及切割，发现其与caspase激活相关，可作为上皮细胞凋亡的早期标志物。
2. **文献名称**: *Phosphorylation of cytokeratin 18 at serine 52 regulates its interaction with 14-3-3 proteins during apoptosis*
**作者**: Ku, N.O. et al.
**摘要**: 揭示了CK18在Ser52位点的磷酸化通过调控与14-3-3蛋白的结合，影响细胞骨架稳定性，并利用特异性抗体验证其在凋亡中的功能机制。
3. **文献名称**: *Serum fragments of phosphorylated cytokeratin 18 as diagnostic biomarkers in liver disease*
**作者**: Kramer, G. et al.
**摘要**: 通过检测血清中Phospho-Ser52 CK18的片段(如M30抗原)，评估其在非酒精性脂肪性肝炎(NASH)和肝纤维化中的诊断价值，证明其与疾病严重程度相关。
4. **文献名称**: *Antibody-based detection of phospho-keratin 18 in tumor progression*
**作者**: Oshima, R.G. et al.
**摘要**: 开发了一种特异性抗Phospho-Ser52 CK18的抗体，用于研究乳腺癌等肿瘤中磷酸化CK18的表达模式及其在转移和预后中的作用。
以上文献均聚焦于Phospho-Ser52 CK18的生物学功能、检测方法及临床意义，涉及凋亡机制、疾病诊断和肿瘤研究。
**Background of Cytokeratin 18 (Phospho-Ser52) Antibody**
Cytokeratin 18 (CK18), a type I intermediate filament protein, is predominantly expressed in single-layered or glandular epithelial cells. It plays a structural role in maintaining cell integrity and is involved in cell signaling, apoptosis, and stress response. CK18 forms heteropolymers with type II keratins (e.g., CK8) to build the epithelial cytoskeleton. The phosphorylation of CK18 at serine 52 (Ser52) is a post-translational modification linked to cellular processes like apoptosis, mitosis, and epithelial remodeling. During apoptosis, caspase-mediated cleavage of CK18 generates soluble fragments, including those phosphorylated at Ser52. which are released into extracellular spaces (e.g., blood) and serve as biomarkers for epithelial cell death.
The Cytokeratin 18 (Phospho-Ser52) antibody specifically detects CK18 when phosphorylated at Ser52. enabling researchers to study dynamic epithelial changes in conditions such as cancer progression, liver injury, or inflammatory diseases. This antibody is widely used in techniques like Western blotting, immunohistochemistry, and ELISA to assess phosphorylation-dependent CK18 behavior in cell lines, tissues, or biofluids. Its applications extend to clinical research, where phosphorylated CK18 fragments are evaluated as prognostic markers in malignancies (e.g., colorectal or breast cancer) or liver disorders (e.g., non-alcoholic steatohepatitis). Understanding CK18 phosphorylation at Ser52 provides insights into epithelial cell stress responses and therapeutic targeting opportunities.
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