| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | BCL6; BCL5; LAZ3; ZBTB27; ZNF51; B-cell lymphoma 6 protein; BCL-6; B-cell lymphoma 5 protein; BCL-5; Protein LAZ-3; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51 |
| Entrez GeneID | 604; |
| WB Predicted band size | 79kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human Bcl-6 around the phosphorylation site of S333. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇与Bcl-6 (Phospho-Ser333)抗体相关的模拟参考文献(内容为示例，非真实文献)：
1. **"Phosphorylation-dependent regulation of Bcl-6 in germinal center B cells"**
- Authors: Dent, A.L.; Shaffer, A.L.; Yu, X.
- 摘要：研究揭示了Bcl-6蛋白在生发中心B细胞中通过Ser333位点磷酸化调控其稳定性的机制，并开发了特异性识别p-Ser333的抗体用于免疫印迹分析。
2. **"BCR signaling induces Bcl-6 phosphorylation at Ser333 to promote transcriptional repression"**
- Authors: Niu, H.; Ye, B.H.; Dalla-Favera, R.
- 摘要：通过BCR信号通路激活的激酶介导Bcl-6 Ser333磷酸化，增强其与共抑制因子的结合能力。文中使用Phospho-Ser333抗体验证了磷酸化在淋巴瘤细胞中的功能。
3. **"Proteasomal degradation of Bcl-6 requires site-specific phosphorylation at Ser333"**
- Authors: Baron, B.W.; Staudt, L.M.
- 摘要：发现Bcl-6的泛素化降解依赖Ser333磷酸化修饰，Phospho-Ser333抗体被用于免疫荧光实验，证实磷酸化与核内蛋白定位变化相关。
(注：以上文献信息为模拟生成，实际引用请查询PubMed或学术数据库。)
The Bcl-6 (Phospho-Ser333) antibody is a specialized tool used to detect the phosphorylated form of B-cell lymphoma 6 (Bcl-6) protein at serine residue 333. Bcl-6 is a transcriptional repressor critical for germinal center formation, lymphocyte differentiation, and immune response regulation. It functions by binding to DNA and recruiting co-repressors to suppress target genes involved in cell cycle control, apoptosis, and inflammatory signaling. Phosphorylation at Ser333. located within its regulatory domain, modulates Bcl-6 activity by influencing its stability, interactions with co-factors, or subcellular localization. This post-translational modification is often linked to cellular signaling pathways such as MAPK or PKC, which are activated during B-cell receptor stimulation or cellular stress.
The antibody enables researchers to study Bcl-6 activation dynamics in physiological contexts like germinal center reactions or pathological conditions such as B-cell lymphomas, where Bcl-6 overexpression or dysregulation is common. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess phosphorylation status in cell lines, tissue samples, or experimental models. Validation typically includes testing in knockout cells or phospho-peptide competition assays to confirm specificity. Understanding Bcl-6 phosphorylation at Ser333 provides insights into its role in immune tolerance, oncogenesis, and potential therapeutic targeting, particularly in diseases driven by aberrant Bcl-6 signaling.
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