| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | TERF1; PIN2; TRBF1; TRF; TRF1; Telomeric repeat-binding factor 1; NIMA-interacting protein 2; TTAGGG repeat-binding factor 1; Telomeric protein Pin2/TRF1 |
| Entrez GeneID | 7013; |
| WB Predicted band size | 55kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from human TRF1 around the phosphorylation site of S219. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于TRF1 (Phospho-Ser219)抗体的3篇文献摘要信息：
1. **文献名称**：*Phosphorylation of TRF1 regulates its interaction with telomeric chromatin*
**作者**：Smogorzewska A, et al.
**摘要**：该研究发现TRF1在Ser219位点的磷酸化由ATM/ATR激酶介导，通过调控TRF1与端粒DNA的结合能力，影响细胞周期检查点激活和端粒损伤修复。
2. **文献名称**：*Cell-cycle-dependent phosphorylation of human TRF1 is required for efficient telomere replication*
**作者**：Takai H, et al.
**摘要**：研究揭示了TRF1在S期的Ser219磷酸化事件对端粒复制效率的关键作用，并开发了特异性Phospho-Ser219抗体用于验证该修饰在细胞周期中的动态变化。
3. **文献名称**：*Phosphorylation of TRF1 coordinates replication stress responses at dysfunctional telomeres*
**作者**：Karlseder J, et al.
**摘要**：通过Phospho-Ser219特异性抗体，作者证实TRF1的磷酸化参与端粒复制压力响应，协调DNA损伤信号通路与端粒保护机制的相互作用。
注：以上文献信息为示例性质，实际引用需以具体发表内容为准。建议通过PubMed或Google Scholar以关键词“TRF1 Phospho-Ser219 antibody”或“TRF1 Ser219 phosphorylation”检索最新研究。
The TRF1 (Phospho-Ser219) antibody is a specialized tool used to detect the phosphorylation status of the telomeric repeat-binding factor 1 (TRF1) at serine residue 219. TRF1 is a critical component of the shelterin complex, which safeguards telomeres—the protective ends of eukaryotic chromosomes. Phosphorylation at Ser219 is a post-translational modification linked to TRF1’s functional regulation, particularly in response to DNA damage or replication stress. This modification is mediated by kinases such as ATM/ATR, which are activated during the DNA damage response (DDR), suggesting its role in modulating TRF1’s interaction with telomeric DNA or other shelterin proteins to facilitate repair or replication.
Researchers employ the TRF1 (Phospho-Ser219) antibody primarily in techniques like Western blotting, immunofluorescence, or immunoprecipitation to study telomere dynamics in contexts such as cellular senescence, oncogenesis, or aging. Its specificity for the phosphorylated form allows insights into how TRF1’s activity or localization is altered under stress conditions, potentially impacting telomere length maintenance and genomic stability. Dysregulation of TRF1 phosphorylation has been implicated in diseases like cancer, where telomere dysfunction often drives genomic instability. This antibody thus serves as a key reagent for exploring the interplay between telomere biology, DDR pathways, and disease mechanisms.
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