| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | KIF20A; MKLP2; RAB6KIFL; Kinesin-like protein KIF20A; GG10_2; Mitotic kinesin-like protein 2; MKlp2; Rab6-interacting kinesin-like protein; Rabkinesin-6 |
| Entrez GeneID | 10112; |
| WB Predicted band size | 100kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from human KIF20A around the phosphorylation site of S528. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于KIF20A (Phospho-Ser528)抗体的3篇参考文献，包含文献名称、作者及摘要内容的简要概括：
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1. **文献名称**：*"KIF20A phosphorylation by PLK1 regulates cytokinesis and its inhibition induces synthetic lethality in BRCA2-deficient cells"*
**作者**：Smith J, et al.
**摘要**：本研究阐明了PLK1对KIF20A Ser528位点的磷酸化在胞质分裂中的关键作用，并开发了一种特异性识别Phospho-Ser528的抗体。实验通过免疫荧光和Western blot验证该抗体的特异性，证明其可用于监测有丝分裂过程中KIF20A的磷酸化动态。
2. **文献名称**：*"Phosphorylation-dependent recruitment of KIF20A to the midbody during abscission"*
**作者**：Chen L, et al.
**摘要**：文章报道了KIF20A Ser528磷酸化是其定位到分裂细胞中间体的必要条件。研究中采用定制Phospho-Ser528抗体，结合质谱和免疫沉淀技术，证实该位点的磷酸化与乳腺癌细胞分裂缺陷相关，抗体特异性通过位点突变实验验证。
3. **文献名称**：*"Development and validation of phospho-specific antibodies for studying mitotic kinase substrates"*
**作者**：Wang Y, et al.
**摘要**：本文系统性描述多种磷酸化抗体(包括KIF20A Ser528)的开发流程。通过肽段免疫和竞争性ELISA验证抗体特异性，并应用于肝癌组织样本分析，揭示KIF20A磷酸化水平与患者预后的相关性。
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**说明**：以上文献为示例性质，实际文献需通过数据库(如PubMed)检索确认。建议结合具体实验需求，关注抗体验证方法(如突变位点对照、功能实验)及疾病模型应用。
The KIF20A (Phospho-Ser528) antibody is a specialized tool used to detect the phosphorylation of serine residue 528 in the KIF20A protein, a kinesin-like motor protein involved in mitotic regulation. KIF20A, also known as MKLP2 (Mitotic Kinesin-Like Protein 2), plays a critical role in cytokinesis by facilitating the transport of vesicles and organelles during cell division. Its activity is tightly regulated by post-translational modifications, including phosphorylation, which modulates its localization and function during mitosis. Phosphorylation at Ser528 is associated with the protein’s interaction with the chromosomal passenger complex (CPC) and its role in forming the midbody during cytokinesis. This modification is thought to occur during late mitosis, potentially mediated by kinases such as Aurora B or PLK1. though specific regulatory mechanisms remain under investigation.
The KIF20A (Phospho-Ser528) antibody is widely used in studies exploring cell cycle dynamics, mitotic errors, and cancer biology, as KIF20A overexpression or dysregulation is linked to tumor progression and poor prognosis in various cancers. Researchers employ this antibody in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess phosphorylation-dependent KIF20A activity in cell lines, tissues, or disease models. Validating its specificity through knockout controls or phosphatase treatment is essential, as cross-reactivity with other phospho-epitopes can occur. Understanding KIF20A phosphorylation provides insights into cell division mechanics and potential therapeutic targets for cancers with mitotic defects.
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