Rabbit Polyclonal Tak1(Phospho-Thr187) Antibody

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     Western Blot analysis of 293T cells using Phospho-Tak1 (T187) Polyclonal Antibody    

The TAK1 (Phospho-Thr187) antibody is a specialized tool used to detect the activated form of Transforming Growth Factor-β-Activated Kinase 1 (TAK1), a key serine/threonine kinase in cellular signaling. TAK1. part of the MAP3K family, regulates pathways like NF-κB and MAPK, influencing processes such as inflammation, apoptosis, and immune responses. Its activation typically occurs through phosphorylation at Thr187 (or Thr184/187 in some isoforms), often triggered by cytokines (e.g., TNF-α, IL-1) or stress signals. This phosphorylation, located in the kinase activation loop, enhances TAK1’s enzymatic activity, enabling it to phosphorylate downstream targets like MKK and IKK complexes, thereby propagating signals critical for cell survival, differentiation, and immune regulation.

The TAK1 (Phospho-Thr187) antibody specifically recognizes this phosphorylated epitope, allowing researchers to study TAK1 activation dynamics in experimental models. It is widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to assess TAK1’s role in diseases like cancer, autoimmune disorders, and chronic inflammation. Dysregulated TAK1 signaling is linked to pathological conditions, making this antibody valuable for investigating therapeutic targets. Validation often includes testing in knockout cells or under phosphorylation-inhibiting conditions to ensure specificity. Understanding TAK1 activation via Thr187 phosphorylation provides insights into cellular stress responses and potential interventions for signaling-related diseases.