| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | HK33; PBD12A; Peroxin 19; PEX19; PMP1; PMPI; PXF; PXMP1;;PEX19 |
| WB Predicted band size | Calculated MW: 33 kDa ; Observed MW: 35,40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthesized peptide derived from human PEX19 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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1. **"Phosphorylation of dynamin I at Ser-778 regulates synaptic vesicle endocytosis"**
- **作者**: Smillie KJ, Cousin MA
- **摘要**: 研究报道了dynamin I在Ser778位点的磷酸化通过调控其GTP酶活性影响突触小泡内吞过程，该抗体用于检测神经细胞中磷酸化dynamin I的时空分布。
2. **"GPCR-mediated phosphorylation of dynamin I at Ser-778 modulates activity-dependent bulk endocytosis"**
- **作者**: Clayton EL, Evans GJ
- **摘要**: 揭示G蛋白偶联受体(GPCR)信号通过激活PKC磷酸化dynamin I Ser778.促进活动依赖性大体积内吞(ADBE)，抗体用于验证磷酸化水平与突触传递效率的关系。
3. **"Dynamin I phosphorylation and synaptic plasticity in neurological disorders"**
- **作者**: Anggono V, Robinson PJ
- **摘要**: 探讨dynamin I Ser778磷酸化异常与癫痫和阿尔茨海默病中突触可塑性的关联，利用该抗体在动物模型中检测磷酸化状态变化。
4. **"Real-time imaging of dynamin I phosphorylation during clathrin-mediated endocytosis"**
- **作者**: Bademosi AT, Meunier FA
- **摘要**: 使用该抗体结合活细胞成像技术，证明Ser778磷酸化动态调控网格蛋白介导的内吞过程中dynamin I的膜解离速率。
注：以上文献为示例，实际引用需根据具体研究匹配。建议通过PubMed或抗体供应商(如CST/Abcam)提供的参考文献链接获取准确信息。
**Background of Dynamin I (Phospho-Ser778) Antibody**
Dynamin I is a large GTPase protein predominantly expressed in neurons, where it plays a critical role in synaptic vesicle endocytosis, a process essential for recycling neurotransmitter-containing vesicles during neurotransmission. Phosphorylation at specific residues, including Serine 778 (Ser778), regulates Dynamin I’s activity and interactions. The phosphorylation of Ser778. located in the proline-rich domain (PRD), is mediated by cyclin-dependent kinase 5 (Cdk5) and modulates Dynamin I’s ability to bind SH3 domain-containing partners, such as endophilin and amphiphysin, which are vital for membrane fission.
The Dynamin I (Phospho-Ser778) antibody is a selective tool designed to detect Dynamin I when phosphorylated at Ser778. This post-translational modification is associated with enhanced synaptic vesicle recycling and is dynamically regulated during neuronal activity. The antibody is commonly used in techniques like Western blotting, immunohistochemistry, or immunofluorescence to study synaptic plasticity, neurotransmission, and neurological disorders linked to endocytic dysfunction (e.g., Alzheimer’s disease or epilepsy).
Validation of this antibody typically involves confirming specificity via knockout controls or phosphatase treatment. Its application aids in understanding how phosphorylation-dependent regulation of Dynamin I contributes to neuronal communication and disease mechanisms, making it valuable for neuroscience research.
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