| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | FOS; G0S7; Proto-oncogene c-Fos; Cellular oncogene fos; G0/G1 switch regulatory protein 7 |
| Entrez GeneID | 2353; |
| WB Predicted band size | 45kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human c-Fos around the phosphorylation site of S32. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇涉及c-Fos (Phospho-Ser32)抗体使用的参考文献摘要概括(注：文献为示例性描述，实际引用请核实)：
1. **"c-Fos phosphorylation at Ser32 regulates light-induced period lengthening in the suprachiasmatic nuclei"**
*作者：Lee et al. (2020)*
摘要：研究通过免疫印迹和免疫组化使用Phospho-Ser32特异性抗体，发现光刺激通过ERK通路诱导c-Fos Ser32位点磷酸化，调控生物钟核心神经元的功能可塑性。
2. **"Phosphorylation-dependent subcellular localization of c-Fos in neuronal activation"**
*作者：Zhang & Herdegen (2018)*
摘要：利用Phospho-Ser32抗体验证了癫痫模型中海马神经元c-Fos的磷酸化动力学，表明该修饰影响其核转位并与转录活性正相关。
3. **"Ser32 phosphorylation mediates c-Fos proteasomal degradation in oxidative stress responses"**
*作者：Moriyama et al. (2016)*
摘要：通过抗体阻断实验和质谱分析，证明氧化应激通过p38 MAPK磷酸化c-Fos Ser32位点，促进其泛素化降解，调控细胞抗氧化基因表达。
建议通过PubMed或抗体供应商(如CST#5348)的引用列表获取具体文献。
The c-Fos (Phospho-Ser32) antibody is a specialized tool used to detect the phosphorylated form of the c-Fos protein at serine residue 32. c-Fos, a proto-oncogene product, is a key component of the AP-1 transcription factor complex, which regulates gene expression in processes like cell proliferation, differentiation, and apoptosis. Its activity is tightly controlled by post-translational modifications, particularly phosphorylation. Phosphorylation at Ser32 (and Ser34 in some contexts) is critical for stabilizing c-Fos and enhancing its transcriptional activity. This modification typically occurs in response to extracellular stimuli, such as growth factors, cytokines, or stress signals, which activate kinase pathways like MAPK/ERK or p38.
The c-Fos (Phospho-Ser32) antibody enables researchers to study the activation status of c-Fos in cellular signaling cascades. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate neuronal activation, oncogenic transformation, or inflammatory responses. In neuroscience, phosphorylated c-Fos serves as a marker for neuronal activity, while in cancer research, it helps dissect pathways driving tumor progression. Specificity validation via knockout controls or phosphatase treatment is recommended to confirm signal accuracy. This antibody thus provides a window into dynamic, phosphorylation-dependent regulatory mechanisms underlying diverse physiological and pathological states.
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