| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | FGFR1; BFGFR; CEK; FGFBR; FLG; FLT2; HBGFR; Fibroblast growth factor receptor 1; FGFR-1; Basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; Fms-like tyrosine kinase 2; FLT-2; N-sam; Proto-oncogene c-Fgr; CD antigen CD331 |
| Entrez GeneID | 2260; |
| WB Predicted band size | 120kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human Flg around the phosphorylation site of Y654. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Flg (Phospho-Tyr654)抗体的参考文献示例(内容为模拟概括，仅供参考)：
1. **"Filaggrin phosphorylation at Tyr654 regulates epidermal barrier homeostasis"**
- **作者**: Smith A, et al.
- **摘要**: 研究探讨了Filaggrin酪氨酸654位点(Tyr654)磷酸化对皮肤屏障功能的影响，利用Phospho-Tyr654抗体证实该修饰通过调控角质化包膜形成参与湿疹病理机制。
2. **"Role of Filaggrin post-translational modifications in keratinocyte differentiation"**
- **作者**: Brown L, et al.
- **摘要**: 通过Phospho-Tyr654抗体检测，发现Tyr654磷酸化在Filaggrin前体分解为功能性单体过程中起关键作用，影响表皮分化信号通路。
3. **"A phospho-specific antibody reveals dysregulated Filaggrin signaling in ichthyosis"**
- **作者**: Chen H, et al.
- **摘要**: 开发并验证了Phospho-Tyr654抗体的特异性，证明其在鱼鳞病患者皮肤样本中检测到异常Filaggrin磷酸化水平，提示与疾病严重程度相关。
4. **"Tyrosine phosphorylation of Filaggrin modulates its interaction with keratin filaments"**
- **作者**: Johnson R, et al.
- **摘要**: 使用Phospho-Tyr654抗体研究Filaggrin与角蛋白丝的结合机制，发现磷酸化修饰通过改变蛋白构象影响细胞骨架稳定性。
(注：以上文献为示例性概括，实际文献需通过学术数据库检索确认。)
Filaggrin (FLG) is a key epidermal protein critical for skin barrier function and hydration. It is synthesized as a large precursor protein, profilaggrin, which undergoes proteolytic processing during keratinocyte differentiation to release functional filaggrin monomers. These monomers aggregate keratin intermediate filaments, facilitating the formation of the cornified envelope in the stratum corneum. Post-translational modifications, including phosphorylation, regulate profilaggrin processing and filaggrin’s interaction with other structural proteins. The phosphorylation of tyrosine 654 (Tyr654) in filaggrin is implicated in modulating its structural and functional roles during epidermal differentiation.
Phospho-Tyr654-specific antibodies are essential tools for studying filaggrin activation, degradation, and its involvement in skin disorders like atopic dermatitis and ichthyosis. Mutations or dysregulation of FLG is linked to impaired barrier integrity and heightened allergen sensitivity. By detecting phosphorylated Tyr654. these antibodies help elucidate signaling pathways influencing profilaggrin processing, such as those involving calcium-dependent proteases or inflammatory cytokines. Researchers also use Phospho-Tyr654 antibodies in immunohistochemistry, Western blotting, or immunofluorescence to assess filaggrin’s spatial-temporal expression in epidermal layers or disease models. Understanding Tyr654 phosphorylation dynamics provides insights into therapeutic strategies targeting skin barrier repair or genetic skin conditions.
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