| WB | 咨询技术 | Human,Mouse,Rat Monkey |
| IF | 咨询技术 | Human,Mouse,Rat Monkey |
| IHC | 咨询技术 | Human,Mouse,Rat Monkey |
| ICC | 1/200-1/1000 | Human,Mouse,Rat Monkey |
| FCM | 咨询技术 | Human,Mouse,Rat Monkey |
| Elisa | 1/40000 | Human,Mouse,Rat Monkey |
| Aliases | CRK; Adapter molecule crk; Proto-oncogene c-Crk; p38 |
| Entrez GeneID | 1398; |
| WB Predicted band size | 40kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat Monkey |
| Immunogen | Synthesized peptide derived from human Crk II around the phosphorylation site of Y221. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇关于Crk II (Phospho-Tyr221)抗体的参考文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**: *"CRK II adaptor protein phosphorylation in BCR-ABL-expressing cells"*
**作者**: Smith J, et al.
**摘要**: 研究BCR-ABL阳性白血病细胞中Crk II蛋白Tyr221位点的磷酸化机制，使用Phospho-Tyr221特异性抗体验证其被ABL激酶激活的过程，并发现磷酸化抑制了Crk II与下游效应蛋白C3G的结合，影响细胞迁移。
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2. **文献名称**: *"Role of Crk II phosphorylation in EGFR signaling and cancer progression"*
**作者**: Tanaka S, et al.
**摘要**: 通过Phospho-Tyr221抗体检测表皮生长因子(EGF)刺激下Crk II的磷酸化动态，证明磷酸化通过调控Crk II/Rap1通路促进肿瘤细胞侵袭，抗体在免疫沉淀和Western blot中表现出高特异性。
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3. **文献名称**: *"Development of a phosphospecific antibody for Crk II Tyr221 and its application in inflammatory disease models"*
**作者**: Lee H, et al.
**摘要**: 报道一种新型Phospho-Tyr221抗体的开发与验证，应用于类风湿性关节炎小鼠模型，显示巨噬细胞中Crk II磷酸化水平升高与炎症信号(如TNF-α)正相关，抗体成功用于组织切片免疫组化分析。
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**备注**：以上文献名为模拟示例，实际研究中建议通过PubMed或SciHub检索关键词“Crk II phosphorylation Tyr221 antibody”获取具体文献。
The Crk II (Phospho-Tyr221) antibody is a specialized tool used to study the phosphorylation status of the adaptor protein Crk II (CT10 regulator of kinase II) at tyrosine residue 221. Crk II, a member of the Crk family of signaling adaptors, plays a critical role in mediating protein-protein interactions involved in cellular processes such as proliferation, migration, and apoptosis. It contains an N-terminal SH2 domain and two SH3 domains, which facilitate its binding to phosphorylated tyrosine residues and proline-rich motifs, respectively. Phosphorylation at Tyr221 (located in the SH3 domain) is a key regulatory mechanism that modulates Crk II's interactions with downstream signaling partners, such as SOS1 or C3G, influencing pathways like Ras-MAPK or JNK signaling.
This phosphorylation event is often triggered by upstream tyrosine kinases, including Abl, EGFR, or Src family kinases, in response to extracellular stimuli (e.g., growth factors, cytokines). Aberrant Crk II activation or phosphorylation has been implicated in cancer progression, inflammation, and fibrosis. The Crk II (Phospho-Tyr221) antibody enables researchers to detect and quantify this post-translational modification using techniques like Western blotting, immunofluorescence, or immunoprecipitation. Its specificity is typically validated using knockout cell lines or peptide competition assays. Studies utilizing this antibody contribute to understanding Crk II's role in disease mechanisms and potential therapeutic targeting of Crk-dependent signaling networks.
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