| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | PTK6; BRK; Protein-tyrosine kinase 6; Breast tumor kinase; Tyrosine-protein kinase BRK |
| Entrez GeneID | 5753; |
| WB Predicted band size | 50kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from human Brk around the phosphorylation site of Y447. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Brk (Phospho-Tyr447)抗体的3篇参考文献的简要概括：
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1. **文献名称**: *"Development and characterization of a phospho-specific antibody for Brk tyrosine kinase at Tyr447"*
**作者**: Lee, H. et al.
**摘要**: 该研究开发了一种特异性识别Brk酪氨酸激酶Tyr447磷酸化位点的多克隆抗体。通过免疫印迹和免疫组化验证了抗体在乳腺癌细胞系中的特异性，并证实该位点磷酸化与Brk的激酶活性及细胞迁移能力相关。
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2. **文献名称**: *"Phosphorylation of Brk tyrosine kinase at Tyr447 promotes epithelial-mesenchymal transition in breast cancer"*
**作者**: Zhang, Y. et al.
**摘要**: 研究发现Brk在Tyr447位点的磷酸化通过激活ERK/MAPK信号通路驱动乳腺癌上皮-间质转化(EMT)。研究中利用抗Phospho-Tyr447 Brk抗体验证了该位点的修饰在肿瘤转移中的作用。
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3. **文献名称**: *"Regulation of PTK6 (Brk) kinase activity by phosphorylation at Tyr447"*
**作者**: Mitchell, P.J. et al.
**摘要**: 本文揭示了Brk(PTK6)在Tyr447位点的自磷酸化对其激酶活性的调控机制。通过特异性抗体检测，作者证明该磷酸化事件可增强Brk与下游底物的结合能力，进而促进癌细胞增殖。
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注：上述文献为示例性内容，实际文献可能需通过学术数据库(如PubMed、Web of Science)检索确认。建议结合具体研究需求进一步筛选。
The Brk (Breast tumor kinase), also known as PTK6. is a non-receptor tyrosine kinase belonging to the SRC family. It plays a role in regulating cell signaling pathways involved in proliferation, differentiation, and migration. Brk is expressed in normal epithelial tissues, such as the skin, gastrointestinal tract, and mammary glands, but is often overexpressed in cancers, including breast, colon, and prostate tumors. Its activity is tightly regulated by phosphorylation events, particularly at specific tyrosine residues.
The Phospho-Tyr447 antibody specifically detects Brk when phosphorylated at tyrosine 447 (Y447), an autophosphorylation site critical for its enzymatic activity and downstream signaling. Phosphorylation at Y447 is associated with Brk activation, enabling interactions with adaptor proteins like Sam68 or IRS-1. which mediate pathways such as MAPK/ERK or PI3K/AKT. This post-translational modification may also influence Brk’s subcellular localization or stability.
Antibodies targeting Brk (Phospho-Tyr447) are essential tools for studying its role in cancer progression, drug resistance, or metastasis. They enable researchers to assess Brk activation status in cell lines or tissues via techniques like Western blotting, immunohistochemistry, or immunofluorescence. Dysregulated Brk signaling has been linked to oncogenic processes, making this antibody valuable for both mechanistic studies and potential therapeutic targeting. Validation of specificity using knockout controls or phosphatase treatment is recommended to ensure accurate detection.
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