| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | EPHB1; ELK; EPHT2; HEK6; NET; Ephrin type-B receptor 1; ELK; EPH tyrosine kinase 2; EPH-like kinase 6; EK6; hEK6; Neuronally-expressed EPH-related tyrosine kinase; NET; Tyrosine-protein kinase receptor EPH-2; EPHB2; DRT; EPHT3; EPTH3; ERK; |
| Entrez GeneID | 2047; |
| WB Predicted band size | 110kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human EphB1/2 around the phosphorylation site of Y594/604. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于 **EphB1/2 (Phospho-Tyr594/604)** 抗体的3篇参考文献的简要总结(注：具体文献可能需要根据实际研究调整，以下为模拟示例)：
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1. **文献名称**: *EphB receptors regulate dendritic spine morphogenesis through Src kinase signaling*
**作者**: Smith A, et al.
**摘要**: 本研究利用 **Phospho-Tyr594/604 EphB1/2 抗体** 验证了 EphB1/B2 受体在神经元突触后位点的酪氨酸磷酸化，发现其通过激活 Src 激酶调控树突棘形态发生，揭示了其在突触可塑性中的作用。
2. **文献名称**: *Phosphorylation-dependent interactions of EphB2 with CrkII promote tumor invasion*
**作者**: Zhang Y, et al.
**摘要**: 通过免疫沉淀和 Western blot 使用 **Phospho-Tyr604 EphB2 抗体**，作者证明 EphB2 在 Tyr604 位点的磷酸化增强了其与适配蛋白 CrkII 的结合，促进肿瘤细胞的迁移和侵袭。
3. **文献名称**: *EphB1 tyrosine phosphorylation in retinal vascular development*
**作者**: Lee H, et al.
**摘要**: 该研究利用 **Phospho-Tyr594 EphB1 抗体** 在小鼠视网膜模型中检测到 EphB1 的磷酸化信号，证实其通过调控 VEGFR2 通路参与血管生成，提示其在眼底疾病中的潜在治疗靶点。
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**注意**：以上文献为示例，实际引用需根据具体研究内容检索 PubMed 或 Google Scholar，建议结合关键词“EphB1 Tyr594 phosphorylation”“EphB2 Tyr604 antibody”等筛选近年研究。抗体厂商(如 CST、Abcam)的产品说明书也可能提供相关参考文献。
The EphB1/2 (Phospho-Tyr594/604) antibody is a specialized tool used to detect phosphorylated tyrosine residues at positions 594 (EphB1) and 604 (EphB2) within these receptor tyrosine kinases (RTKs). Eph receptors, including EphB1 and EphB2. are part of the Eph-ephrin signaling system, which plays critical roles in cell-cell communication, tissue patterning, and developmental processes such as axon guidance, angiogenesis, and synaptic plasticity. Tyrosine phosphorylation at these specific residues occurs during receptor activation, typically triggered by binding to ephrin-B ligands. This post-translational modification facilitates downstream signaling cascades involved in cell adhesion, migration, and cytoskeletal reorganization.
Dysregulation of EphB1/B2 signaling has been implicated in cancer progression, neurological disorders, and vascular diseases. The Phospho-Tyr594/604 antibody enables researchers to study the activation status of EphB1/B2 in physiological and pathological contexts, providing insights into signaling dynamics. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess receptor activation in cell lines, tissues, or disease models. Specificity for the phosphorylated forms ensures accurate detection of active EphB1/B2. aiding in the exploration of therapeutic targets or biomarkers associated with Eph receptor dysfunction.
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